| 人卵巢癌顺铂耐药细胞EZH2基因沉默对Drosha基因表达的影响 |
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| 引用本文： | 况燕,蔡晶,李桃,李东林,周隽,王泽华.人卵巢癌顺铂耐药细胞EZH2基因沉默对Drosha基因表达的影响[J].广西医学,2012,34(6):660-662. |
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| 作者姓名： | 况燕 蔡晶 李桃 李东林 周隽 王泽华 |
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| 作者单位： | 况燕 (广西医科大学第一附属医院妇产科,南宁市,530021) ； 蔡晶 (华中科技大学同济医学院附属协和医院妇产科,武汉市,430022) ； 李桃 (华中科技大学同济医学院附属协和医院妇产科,武汉市,430022) ； 李东林 (华中科技大学同济医学院附属协和医院妇产科,武汉市,430022) ； 周隽 (华中科技大学同济医学院附属协和医院妇产科,武汉市,430022) ； 王泽华 (华中科技大学同济医学院附属协和医院妇产科,武汉市,430022) ； |
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| 摘 要： | 目的探讨RNA干扰沉默果蝇zeste基因增强子同源物2(EZH2)基因对卵巢癌获得性耐药细胞株A2780/DDP的Drosha基因表达的影响。方法获得稳定表达EZH2短发夹状RNA(shRNA)的人卵巢癌获得性耐药细胞株A2780/DDP-shEZH2,实时荧光定量PCR法和免疫印记法检测转染株的Drosha基因表达。结果以未转染组A2780/DDP细胞Drosha的表达水平为100.00%,EZH2-shRNA转染组A2780/DDP-shEZH2细胞Drosha的mRNA及蛋白水平分别上升(53.33±9.24)%和(51.28±3.25)%。结论沉默EZH2基因可以上调Drosha基因的表达。
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| 关 键 词： | 卵巢肿瘤 EZH2基因 Drosha基因 MicroRNA 抗药性 |
Effect of Silencing EZH2 on Drosha Gene Expression in Cisplatin-resistant Ovarian Cancer Cell Line A2780/DDP |
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| KUANG Yan,CAI Jin,LI Tao,LIN Dong-lin,ZHOU Jun,WANG Ze-hua.Effect of Silencing EZH2 on Drosha Gene Expression in Cisplatin-resistant Ovarian Cancer Cell Line A2780/DDP[J].Guangxi Medical Journal,2012,34(6):660-662. |
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| Authors: | KUANG Yan CAI Jin LI Tao LIN Dong-lin ZHOU Jun WANG Ze-hua |
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| Institution: | (Department of Obstetrics and Gynecology,Union Hospital,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022,China) |
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| Abstract: | Objective To investigate the effect of gene silencing of RNA interfering enhancer of zeste homolog 2(EZH2) on Drosha gene expression in cisplatin-resistant ovarian cancer cell line A2780/DDP.Methods After the silencing EZH2 A2780/DDP cell line was obtained by short hairpin RNA(shRNA) technique,the mRNA and protein expression of Drosha were detected by real-time qRT-PCR and Western blot.Results The Drosa expression of A2780/DDP in untransfection group was 100%,and the expressions of Drosha mRNA and protein in EZH2-shRNA transfection group increased by(53.33±9.24)% and(51.28±3.25)%,respectively.Conclusion Silencing EZH2 can increase the expression of Drosha gene. |
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| Keywords: | Ovarian neoplasm Enhancer of zeste homolog 2 gene Drosha gene MicroRNA Drug resistance |
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