| 双孔钾通道TREK-1在神经细胞正常及缺氧条件下的表达研究 |
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| 引用本文： | 吴潇,谢敏杰,唐荣华. 双孔钾通道TREK-1在神经细胞正常及缺氧条件下的表达研究[J]. 神经损伤与功能重建, 2012, 7(3) |
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| 作者姓名： | 吴潇 谢敏杰 唐荣华 |
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| 作者单位： | 吴潇 (华中科技大学同济医学院附属同济医院神经内科,武汉,430030) ； 谢敏杰 (华中科技大学同济医学院附属同济医院神经内科,武汉,430030) ； 唐荣华 (华中科技大学同济医学院附属同济医院神经内科,武汉,430030) ； |
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| 摘 要： | 目的:研究双孔钾通道TREK-1在神经元及星形胶质细胞的表达,及在缺氧时星形胶质细胞中的表达变化。方法:离体培养大鼠皮质神经元和星形胶质细胞,利用免疫荧光染色法观察TREK-1蛋白在神经元和星形胶质细胞的表达分布,对星形胶质细胞进行缺氧干预,实时PCR观察缺氧不同时间TREK-1的表达变化。结果:TREK-1通道在神经元和星形胶质细胞均有表达,缺氧时TREK-1在星形胶质细胞中呈先上升,后逐渐下降的趋势。结论:TREK-1在神经元和星形胶质细胞中均有表达,星形胶质细胞中TREK-1的表达在缺氧损伤中发生动态变化。
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| 关 键 词： | TREK-1 神经元 星形胶质细胞 缺氧 |
Expression of TREK-1 on Cultured Neuron and Astrocytes under Normal and Hypoxia Condition |
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| Abstract: | Objective:To investigate the expression of two-pore domain potassium channel—TREK-1 on primary cultured neurons and astrocytes and the temporal changes of TREK-1 expression in astrocytes under hypoxia insult.Methods: The cortical neurons and astrocytes were cultured.The expression of TREK-1 in cultured neurons and astrocytes were analyzed by double immunofluorescence staining.The temporal changes of TREK-1 expression in astrocytes under hypoxia insults were detected by Real-time PCR.Results: The TREK-1 immuno-reactivity was expressed on both the neurons and astrocytes under normal condition.During hypoxia insult,the TREK-1 expression on astrocyte increases at early phases and decreases gradually at late phase.Conclusion: The TREK-1 immunoreactivity is expressed on both the neurons and astrocytes.In astrocytes under hypoxia,there are temporal changes on TREK-1 expression. |
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| Keywords: | TREK-1 neuron astrocyte hypoxia |
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