梅毒孕妇产后血清固定者的细胞免疫研究

目的了解梅毒孕妇产后血清固定者的细胞免疫水平。方法 60例梅毒产妇规范治疗后血清梅毒甲苯胺红不加热血清试验(TRUST)持续低滴度阳性(滴度≤1∶4,时间>2年)且排除HIV感染和神经梅毒者作为病例组,并选取61例治疗后TRUST转阴的女性梅毒患者和63例女性健康体检者作对照组,采用流式细胞仪检测外周血CD3、CD4、CD8、辅助性T细胞17(Th17)细胞和酶联免疫吸附法(ELISA)检测Th17细胞相关细胞因子(IL-17和IL-23)。结果病例组外周血CD3和CD4淋巴细胞亚群均低于2个对照组(P<0.001),而CD8则高于2个对照组(P<0.05);3组的Th17淋巴细胞亚群、血IL-17和IL-23的值相比差异无统计学意义(P>0.05)。结论梅毒孕妇产后血清固定患者存在细胞免疫异常。

Study on Cellular Immunity in Pregnant Women Infected With Syphilis Undergoing Seroresistance After Delivery

Objective To investigate cellular immunity in pregnant women infected with syphilis undergoing seroresistance after delivery.Methods Sixty pregnant women infected with syphilis after delivery,with positive toluidine red unheated serum test(TRUST) at low titre(titre≤1 ∶4,duration>2 years) and little possibility of HIV infection and neurosyphilis,were enrolled into the study as case group,while 61 syphilis women with negative TRUST after treatment and 63 healthy women as two control groups.CD3,CD4,CD8 and T helper cell(Th17) lymphocyteswere measured with flow cytometry(FCM),and IL-17,IL-23 with enzyme-linked immuno-sorbent assay(ELISA).Results CD3,CD4 lymphocyte subsets in case group were significantly lower than those in other two groups(P<0.001),but CD8 lymphocyte subsets in case group was higher than that in other 2 groups(P<0.05);There was no significant difference in Th17 lymphocyte subsets,IL-17 and IL-23 among the three groups(P>0.05).Conclusion Abnormality of cellular immunity can be found in pregnant women infected with syphilis undergoing seroresistance after delivery.