高压氧联合抗震颗粒与美多芭对帕金森病大鼠黑质Bcl-2、Bax影响的对比研究

目的对比研究高压氧联合抗震颗粒与美多芭对实验性帕金森病大鼠黑质Bcl-2、Bax表达的影响及意义。方法采用单点注射6-羟基多巴胺(6-OHDA)建立偏侧帕金森病大鼠模型18只,随机分为高压氧联合抗震颗粒组6只,美多芭组6只,模型组6只,给药后14 d,采用免疫组织化学法检测各组大鼠大脑黑质Bcl-2、Bax的表达,并观察各组大鼠治疗前后有无异常行为。结果高压氧联合抗震颗粒组与美多芭组的Bcl-2、Bax蛋白阳性表达率比较差异均无统计学意义(P>0.05);高压氧联合抗震颗粒组及美多芭组Bcl-2蛋白阳性表达率明显高于模型组,Bax蛋白阳性表达率明显低于模型组(P<0.05)。结论高压氧联合抗震颗粒与美多芭均能抑制帕金森病大鼠黑质细胞凋亡,对帕金森病大鼠有治疗作用。

Comparison Study on the Effect of Hyperbaric Oxygen Combined With Anti-parkinson Granule,Levodopa and Benserazide Hydrochloride Tablets on the Change of Expression of Bcl-2 and Bax Protein in Parkinsonian Rats

Objective To compare the effects of hyperbaric oxygen combined with anti-parkinson granule,levodopa and benserazide hydrochloride tablets on the expression of Bcl-2 and Bax protein in experimental parkinsonian rats.Methods The parkinsonian rat model was established with injection of 6-hydroxydopamine(6-OHDA) into the ventral tegmental of right side brain.The 18 model rats were randomly divided into 3 groups:the treatment group by using hyperbaric oxygen combined with anti-parkinson granule(n=6),the treatment group by using levodopa and benserazide hydrochloride tablets(n=6) and the model group(n=6).After the subjects were treated for 14 days,expression of Bcl-2 and Bax protein in parkinsonian rats were detected by immunohistochemistry assay,and the changes of ethology were observed in the 3 groups.Results No significant difference was found in the positive expressions of Bcl-2 and Bax protein between the two treatment groups(P>0.05).The positive expressions of Bcl-2 were significantly increased,and the positive expressions of Bax protein were decreased in the two treatment groups compared with those in the model groups(P<0.05).Conclusion Hyperbaric oxygen combined with anti-parkinson granule,can significantly restrain apoptosis of substantia nigra as well as levodopa and benserazide hydrochloride tablets,and shows a good curative effect in the treatment of parkinsonian rats.