缺血后处理对脑缺血再灌注损伤后ERK1/2和Akt磷酸化及神经细胞凋亡的影响 |
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引用本文: | 邢变枝,陈晖,张苏明.缺血后处理对脑缺血再灌注损伤后ERK1/2和Akt磷酸化及神经细胞凋亡的影响[J].国外医学:物理医学与康复学分册,2012,7(3). |
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作者姓名: | 邢变枝 陈晖 张苏明 |
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作者单位: | 邢变枝 (武汉大学人民医院放射科,武汉,430060) ; 陈晖 (武汉大学人民医院泌尿外科,武汉,430060) ; 张苏明 (华中科技大学同济医学院附属同济医院神经内科,武汉,430030) ; |
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摘 要: | 目的:观察缺血后处理对大鼠局灶性脑缺血再灌注损伤后ERK1/2和Akt及神经细胞凋亡的影响。方法:成年健康SD大鼠72只,随机分为假手术(sham)组、缺血再灌注(I/R)组、缺血后处理(Postcond)组各24只,应用线栓法建立大脑中动脉闭塞(MCAO)再灌注模型。分别于再灌注10min、30min、6h、24h后留取大脑皮质。Western blot检测再灌注10min、30min、6h后ERK1/2和Akt活性变化;原位末端标记(TUNEL)检测再灌注后24h神经细胞凋亡。结果:Postcond组再灌注10min、30min、6h后ERK1/2和Akt活性高于I/R组(P<0.05);脑缺血再灌注24h后,Postcond组与I/R组比较,TUNEL阳性细胞减少(P<0.05)。结论:缺血后处理可提高大鼠脑缺血再灌注后皮质内ERK1/2和Akt活性,减少神经细胞凋亡。
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关 键 词: | 缺血后处理 ERK1/2 Akt 脑缺血再灌注损伤 |
Effects of Ischemic Post-condtioning on Activation of ERK1/2 and Akt and Neuronal Apoptosis in Rats with Cerebral Ischemia Reperfusion Injury |
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Abstract: | Objective: To investigate the effects of ischemic post-condtioning on ERK1/2 and Akt activation and neuronal apoptosis in rats with cerebral ischemia reperfusion injury.Methods: SD rat with focal ischemic reperfusion was induced by intraluminal middle cerebral artery occlusion(MCAO) with a nylon monofilament suture.Ischemic animals were randomly assigned to 3 groups(n=24),the sham group,I/R group and Postcond group.In Postcond group,the animals with MCAO were further subject to postconditioning(6 cycles of 30s ischemia and 30s reperfusion) after MCAO.The phosphorylation of Akt and ERK1/2 in the cortex was measured by Western Blot.The neuronal apoptosis was detected by TUNEL staining.Results: Postcond treatment significantly increased phosphorylation of Akt and ERK1/2 at 10 min,30 min,and 6 h after reperfusion.At 24 h after reperfusion,the rats in the Postcond group showed much less TUNEL positive cells in the cortex than those in the I/R group.Conclusion:Ischemic post-conditioning decreases apoptosis and improves neurological functions,perhaps which by increasing levels of Akt and ERK1/2 activation in cerebral I/R injury. |
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Keywords: | ischemic post-condtioning ERK1/2 Akt cerebral ischemia reperfusion injury |
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