| 应用多细胞系微量细胞培养法分离脑炎病毒 |
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| 引用本文： | 韩文清,宫连凤,刘娟,张萍,孙颖,李鹏,万言珍,孟红. 应用多细胞系微量细胞培养法分离脑炎病毒[J]. 医学动物防制, 2013, 0(2): 168-169 |
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| 作者姓名： | 韩文清 宫连凤 刘娟 张萍 孙颖 李鹏 万言珍 孟红 |
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| 作者单位： | 烟台市疾病预防控制中心;山东省医学科学院 |
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| 摘 要： | 目的探索应用多细胞系微量细胞培养法进行病脑脑脊液病毒分离,以建立简便、快速的病毒初筛方法。方法采集病毒性脑炎病例脑脊液46份,应用4种细胞系在96孔细胞培养板进行分离病毒,所得培养物用肠道病毒通用引物RT-PCR扩增,进行初步鉴定。结果 46份标本分离病毒11株,其中3株应用肠道病毒通用引物扩增出预期片段。结论多细胞系微量细胞培养法具有快速、便捷的特点,是一种较好的病毒初筛方法。
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| 关 键 词： | 病毒性脑炎 分离鉴定 脑脊液 |
Isolating virus of encephalitis with muti-cells and micro-plate method |
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| HAN Wen-qing,GONG Lian-feng,LIU Juan,ZHANG Ping,SUN Ying, LI Peng,WAN Yan-zhen,MENG Hong. Isolating virus of encephalitis with muti-cells and micro-plate method[J]. Chinese Journal of Pest Control, 2013, 0(2): 168-169 |
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| Authors: | HAN Wen-qing GONG Lian-feng LIU Juan ZHANG Ping SUN Ying LI Peng WAN Yan-zhen MENG Hong |
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| Affiliation: | Yantai Center for Disease Control and Prevention,Shandong 264003,China. |
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| Abstract: | Objective Isolating virus from cerebrospinal fluids applying multi-cells and micro-plate method to build a simple and convenient method to initial screen virus.Method Collect 46 cerebrospinal fluids(CSF)from the viral encephalitis cases and isolate virus applying 4 cells in 96-well-microplate.The culture obtained were amplified with intestinal virus universal primer RT-PCR and identified preliminarily.Results 11 strains were isolated from 46 specimens,and 3 strains were amplified using consensus primers for enterovirus.Conclusions Multi-cells and micro-plate method was a convenient,simple and better method for initial screen virus. |
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| Keywords: | Viral encephalitis Isolation and Identification Cerebrospinal fluids |
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