| Coculture of elongated neuron axon with poly （D, L-lactide-co-glycolide） biomembrane in vitro |
| |
| 引用本文： | 程飚,陈峥嵘. Coculture of elongated neuron axon with poly （D, L-lactide-co-glycolide） biomembrane in vitro[J]. 中华创伤杂志(英文版), 2004, 7(4): 217-219 |
| |
| 作者姓名： | 程飚 陈峥嵘 |
| |
| 作者单位： | DepartmentofOrthopaedics,ZhongshanHospital,FudanUniversity,Shanghai200032,China |
| |
| 基金项目： | FundedbyShanghaiBoardofHealthgrant (2 0 0 3 97)andShanghaiScienceDevelopmentFundation (No .0 14 0 190 2 8) |
| |
| 摘 要： | Objective: To elongate human nerve axon in culture and search for suitable support matrices for peripheral nervous system transplantation. Methods: Human embryo cortical neuronal cells, seeded on poly (D, L-lactide-co-glycolide) (PLGA) membrane scaffolds, were elongated with a self-made neuro-axon extending device. The growth and morphological changes of neuron axons were observed to measure axolemmal permeability after elongation. Neurofilament protein was stained by immunohistochemical technique. Results: Human embryo neuron axon could be elongated and cultured on the PLGA membrane and retain their normal form and function. Conclusions: Three dimensional scaffolds with elongated neuron axon have the basic characteristics of artificial nerves, indicating a fundemental theory of nerve repair with elongated neuron axon.
|
| 关 键 词： | 协调细胞培养 神经细胞延长 轴突 聚乙稀 D L-丙交酯-co-醣脂类 生物膜 体外 PLGA |
Coculture of elongated neuron axon with poly (D, L-lactide-co-glycolide) biomembrane in vitro |
| |
| CHENG Biao and CHEN Zheng-rong. Coculture of elongated neuron axon with poly (D, L-lactide-co-glycolide) biomembrane in vitro[J]. Chinese journal of traumatology, 2004, 7(4): 217-219 |
| |
| Authors: | CHENG Biao and CHEN Zheng-rong |
| |
| Affiliation: | Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China |
| |
| Abstract: | Objective: To elongate human nerve axon in cultu re and search for suitable support matrices for peripheral nervous system trans plantation. Methods: Human embryo cortical neuronal cells,seeded on poly ( D,L-lactide-co-glycolide) (PLGA) membrane scaffolds,were elongated with a se lf-made neuro-axon extending device. The growth and morphological changes of n euron axons were observed to measure axolemmal permeability after elongation. Ne urofilament protein was stained by immunohistochemical technique.Results: Human embryo neuron axon could be elongated and cultur ed on the PLGA membrane and retain their normal form and function. Conclusions: Three dimensional scaffolds with elongated neuron axon have the basic characteristics of artificial nerves,indicating a fundement al theory of nerve repair with elongated neuron axon. |
| |
| Keywords: | Axons Cells culture Tissue engineering |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
