| A型流感病毒M基因DNA疫苗载体构建及免疫性研究 |
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| 引用本文： | 吴春利,程小雯,吕星,房师松,王昕.A型流感病毒M基因DNA疫苗载体构建及免疫性研究[J].中国热带医学,2011,11(7):800-802. |
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| 作者姓名： | 吴春利 程小雯 吕星 房师松 王昕 |
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| 作者单位： | 深圳市疾病预防控制中心,广东深圳,518055 |
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| 摘 要： | 目的构建A型流感病毒M基因DNA疫苗质粒载体并对免疫后小鼠体内免疫应答反应进行分析和评价。方法将H3N2亚型流感病毒M基因通过RT-PCR扩增后克隆构建DNA疫苗载体pBudCE4．1-M，并将pBudCE4．1-M质粒体外瞬时转染COS7细胞并通过Western blot鉴定M1蛋白的表达，然后将pBudCE4．1-M质粒免疫小鼠。通过EUSA对小鼠体内生成的M1蛋白IgG抗体进行鉴定。结果M基因被成功克隆到质粒载体pBudCE4．1上，并在体外COS7细胞中表达出28kD的M1蛋白。疫苗质粒免疫小鼠后4周，在小鼠血清中检测到M1蛋白kG抗体的吸光度有明显升高。结论成功构建了一种A型流感病毒M基因DNA疫苗载体，将其免疫小鼠后成功诱导小鼠体内产生了特异性的IgG抗体。
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| 关 键 词： | A型流感病毒 M基因 DNA疫苗 血清抗体 |
Construction of M gene DNA vaccine vectors of influenza A virus and its immune response in vivo |
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| Institution: | WU Chun-li,CHENG Xiao-wen,Lü Xing,et al.(Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,Guangdong,P.R.China) |
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| Abstract: | Aim To develop M gene DNA vaccine vectors of influenza A virus and evaluate its immune response in vivo.Methods Amplified by RT-PCR,Mgene(H3N2)were cloned into vector pBudCE4.1.Then the recombinant plasmid pBudCE4.1-M was transfected transiently into COS7 cells and the expression of M1 protein was tested by western blot.Mice were immunized with the plasmid pBudCE4.1-M and the M1-specific antibody was detected by ELISA.Results The M gene was cloned into plasmid pBudCE4.1 successfully and the 28kD M1 proteins... |
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| Keywords: | Influenza Avirus Mgene DNA vaccine Serum antibody |
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