| 临床难鉴定细菌和真菌核糖体基因扩增及序列分析 |
| |
| 引用本文： | 吴奎海,孙静静,王前,芮勇宇.临床难鉴定细菌和真菌核糖体基因扩增及序列分析[J].中国热带医学,2011,11(9):1053-1055. |
| |
| 作者姓名： | 吴奎海 孙静静 王前 芮勇宇 |
| |
| 作者单位： | 南方医科大学南方医院检验科,广东广州,510515 |
| |
| 摘 要： | 目的建立核糖体测序方法鉴定临床难鉴定的细菌和真菌。方法分别利用通用引物扩增细菌16S rRNA基因和真菌核糖体转录间隔区2（ITS2）,对PCR产物进行测序,在GenBank中对测序结果进行Blastn等分析。结果21株临床常见细菌和8株真菌测序结果与表型鉴定符合,检测出6株临床难鉴定细菌和真菌。结论核糖体测序可以作为临床难鉴定细菌和真菌的分子生物学诊断方法。
|
| 关 键 词： | 细菌 真菌 16SrRNA基因 转录间隔区 |
Amplification of ribosomal sequence of clinical unidentifiable bacteria and fungi and sequence analysis |
| |
| WU Kui-hai,SUN Jing-jing,WANG Qian,et al..Amplification of ribosomal sequence of clinical unidentifiable bacteria and fungi and sequence analysis[J].China Tropical Medicine,2011,11(9):1053-1055. |
| |
| Authors: | WU Kui-hai SUN Jing-jing WANG Qian |
| |
| Institution: | WU Kui-hai,SUN Jing-jing,WANG Qian,et al.(Department of Clinical Laboratory,NanFang Hospital,Southern Medical University,Guangzhou 510515,China |
| |
| Abstract: | Objective To establish a ribosome sequencing method for idnentifying clinical unidentifiable bacteria and fungi.Methods Amplification and sequencing of 16S rRNA gene of bacteria and internal transcribed spacer 2(ITS2)region of fungi were performed using respective universal primers.Searches of current nucleotide databases in GenBank were carried out with the BLAST algorithm.Results There 21 clinical common bacterial and 8 fungal isolates were accurately identified.Six clinical rare bacterial and fungal isol... |
| |
| Keywords: | Bacteria Fungi 16S rRNA gene Internal transcribed spacer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
