海南地不容总碱的镇痛抗炎作用及毒性观察

目的研究海南地不容总生物碱的镇痛抗炎作用及急性毒性。方法采用小鼠热板法、扭体法及甲醛测痛试验观察镇痛作用；采用二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性、棉球诱导小鼠肉芽组织增生试验模型观察抗炎作用，通过胸腔白细胞游走试验研究其对炎症渗出液中白细胞数量的影响，应用交叉菜胶致足肿胀试验测定炎性组织中前列腺素E2（PGE2）含量并初步探讨其抗炎机理，急性毒性试验采用改良寇氏法测定LD50。结果海南地不容总碱能明显延长小鼠舔后足时间，减少扭体次数，降低甲醛测痛试验第一、二时相的疼痛强度；减轻小鼠耳肿胀，抑制毛细血管通透性增高及肉芽组织增生，有效抑制白细胞游走，并能明显减少大鼠炎症组织中PC-E2含量。其LD50为0．569g·kg-1，95％可信限为0．519～0．623g·kg-1。结论海南地不容总碱镇痛、抗炎作用显著，其机理可能与抑制PGE2的产生与释放有关。

Analgesic and anti-inflammatory effects of total alkaloids from Stephania hainanensis in Hainan and it's acute toxicity

Aim To study the analgesic and anti-inflammatory effects of total alkaloids from Stephania hainanensis H.S.Lo et Y.Tsoong in Hainan and it's acute toxicity in mice.Methods The analgesic effects were evaluated by the hot-plate test,twisting reaction induced by acetic acid,and formalin test.The anti-inflammatory activity was tested by several typical inflammatory models involving dimethylbenzene-induced ear swelling in mice,capillary penetration method,cotton pellet granuloma.The anti-inflammatory mechanism w...