| 脂多糖活化小鼠大脑小胶质细胞后炎性细胞因子的表达 |
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| 引用本文： | 辛岗,苏芸,许燕璇,陈小璇,李康生. 脂多糖活化小鼠大脑小胶质细胞后炎性细胞因子的表达[J]. 中国热带医学, 2011, 11(8): 983-984,988 |
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| 作者姓名： | 辛岗 苏芸 许燕璇 陈小璇 李康生 |
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| 作者单位： | 汕头大学医学院微生物与免疫学教研室,广东省高校免疫病理重点实验室,广东,汕头,515041 |
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| 基金项目： | 广东省医学科研基金(No.A2010392); 国家自然科学基金(No.30771988); 汕头市重点科技计划项目(No.汕府科[2009]70号-200903) |
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| 摘 要： | 目的采用细菌脂多糖（LPS）对原代培养的BALB/c小鼠大脑皮质小胶质细胞进行刺激,检测小胶质细胞IL-1bI、L-6和TNF-α等细胞因子的基因表达情况。方法分离纯化小胶质细胞,采用1μg/ml LPS刺激24h,再采用RT-PCR检测IL-1bI、L-6和TNF-a mRNA表达水平。结果体外培养的小胶质细胞经LPS刺激后,细胞因子IL-1bI、L-6和TNF-a mRNA表达水平明显上调。结论 LPS活化的小胶质细胞可产生大量的炎性细胞因子。
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| 关 键 词： | 脂多糖 小胶质细胞 白细胞介素-1b 白细胞介素-6 肿瘤坏死因子-a |
Expression of IL-1,IL-6 and TNF-by primary cultured cortical microglia after treated with bacterial lipopolysaccharide |
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| XIN Gang,SU Yun,XU Yan-Xuan,et al.. Expression of IL-1,IL-6 and TNF-by primary cultured cortical microglia after treated with bacterial lipopolysaccharide[J]. China Tropical Medicine, 2011, 11(8): 983-984,988 |
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| Authors: | XIN Gang SU Yun XU Yan-Xuan et al. |
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| Affiliation: | XIN Gang,SU Yun,XU Yan-Xuan,et al.(Department of Microbiology and Immunology,Shantou University Medical College,Shantou 515041,Guangdong,P.R.China) |
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| Abstract: | Objective To investigate the productions of inflammatory cytokines by primary cultured BALB/c cortical microglia after being treated with LPS.Methods The purified microglia was cultured and then treated by LPS for 24h.The gene expressions of IL-1,IL-6 and TNF-were detected by RT-PCR.Results Compared with the untreated control group,the active microglia could express high levels of IL-1,IL-6 and TNF-mRNA after treatment with LPS(P<0.05).Conclusion After being stimulated by LPS,the microglia could be activate... |
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| Keywords: | Lipopolysacchride Microglia Interleukin-1 Interleukin-6 Tumor necrosis factor-α |
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