克罗卡林及优降糖对实验性急性心肌梗死的影响

目的：研究克罗卡林及优降糖对心肌梗死面积及梗死部位心肌细胞三磷酸腺苷（ＡＴＰ）含量的影响。方法：以Ｗｉｓｔａｒ大鼠为模型，开胸安置缺血－再灌注装置，将大鼠分为４组，Ａ组未作特殊处理；Ｂ组阻断冠状动脉（冠脉）１小时，再灌注２小时；Ｃ组冠脉阻断前０．５小时静注克罗卡林１００μｇ／ｋｇ，Ｄ组冠状阻断前０．５９胸注克罗卡林１００μｇ／ｋｇ，接着予优降糖０．３ｍｇ／ｋｇ。各组动物经上述处理后，抽股静脉血侧肌

Effect of cromakalim and glibenclamide on infarct size and ATP content of myocardium in experimental rat model of acute myocardial infarction

Objective:To study the effect of cromakalim and glibenclamide on infarct size and ATP content of myocardium in rats exposed to ischemiareperfusion (I/R).Methods:The snare for occlusion and reperfusion was blaced to the main coronary artery in 84 Wistar rats which were divided into 4 groups:control,I/R, I/R cromakalim and I/R cromakalim glibenclamide.Animals in each group sustained ischemia for 1 hour followed by 2 hours of reperfusion.The plasma concentration of creatine kinase and the tissue ATP content of myocardium were assayed and the infarct size of myocardium was measured.Results:The protective effect of cromakalim was evident.The infarct size and the plasma concentration of CK were lower while the tissue content of ATP was much higher than those of I/R group ( P <0 01).Glibenclamide reduced the effect of cromakalim ( P <0 01).Conclusions:Cromakalim protects the myocardium against I/R damage,the possible mechanism of which is related to the activation of the ATPsensitive potassium channel of myocardial membrane.