| RP-HPLC法测定迷迭香中鼠尾草酸和迷迭香酸的含量 |
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| 引用本文： | 张坤,许秋雁,叶小燕,谢朝阳,郑群怡.RP-HPLC法测定迷迭香中鼠尾草酸和迷迭香酸的含量[J].中南药学,2013(8):603-605. |
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| 作者姓名： | 张坤 许秋雁 叶小燕 谢朝阳 郑群怡 |
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| 作者单位： | 康宝菜蕾硕湖南天然产物有限公司,长沙410100 |
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| 基金项目： | 湖南省科技计划重大专项（编号：2010FJ1010） |
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| 摘 要： | 目的建立高效液相色谱法同时测定迷迭香药材中鼠尾草酸和迷迭香酸的含量。方法运用PhenomenexC18（250 mm×4.60 mm,5μm）为分析柱;柱温30℃;流动相为乙腈（A）-0.1%磷酸（B）梯度洗脱：0～10min,25%A,10～15 min：25%～75%A,15～25 min：75%A;流速1.0 mL min^-1;检测波长284 nm。结果迷迭香酸和鼠尾草酸与其他组分的分离效果好,两者分别在0.046 55～1.862 0μg和0.372 7～14.908μg呈良好的线性关系（r值为0.999 94和0.999 97）,平均回收率分别为99.0%（RSD=1.1%,n=9）和98.1%（RSD=1.5%,n=9）。结论该测定方法简便、准确、重现性好,方便了迷迭香药材中鼠尾草酸和迷迭香酸的含量测定。
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| 关 键 词： | 迷迭香 迷迭香酸 鼠尾草酸 反相高效液相色谱法 |
Determination of rosmarinic acid and carnosic acid in Rosmarinus officinalis L. by RP-HPLC |
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| ZHANG Kun,XU Qiu-yan,YE Xiao-yan,XIE Zhao-yang,ZHENG Qun-yi.Determination of rosmarinic acid and carnosic acid in Rosmarinus officinalis L. by RP-HPLC[J].Central South Pharmacy,2013(8):603-605. |
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| Authors: | ZHANG Kun XU Qiu-yan YE Xiao-yan XIE Zhao-yang ZHENG Qun-yi |
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| Institution: | (Herbalife NatSource(Hunan),Natural Products Co.,Ltd.,Changsha 410100) |
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| Abstract: | Objective To establish an HPLC method to determine the content of rosmarinic acid and carnosic acid in Rosmarinus officinalis L.Methods Phenomenex C18（250 mm×4.60 mm,5 μm） at 30 ℃ was used.The mobile phase was acetonitrile（A）-0.1% phosphoric（B）： 0-10 min： 25%A;10-15 min： 25%-75%A;and 15-25 min： 75%A.The flow rate was 1.0 mL min^-1.The detection wavelength was 284 nm.Results Rosmarinic acid and carnosic acid were well isolated from other components,and the calibration curve showed a good linear correlation at 0.046 55-1.86 20 μg and 0.372 7 14.908 μg.The average recovery was 99.0%（RSD = 1.1%,n = 9） and 98.1%（RSD = 1.5%,n = 9）.Conclusion The method is simple,accurate and reliable for the determination of rosmarinic acid and carnosic acid in Rosmarinus officinalis L. |
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| Keywords: | Rosmarinus officinalis L rosmarinic acid carnosic acid RP-HPLC |
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