| 高效液相色谱法测定复方石淋通胶囊中夏佛塔苷的含量 |
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| 引用本文： | 马杰,童珂,罗晖明,丁野,胡娟妮,李文莉.高效液相色谱法测定复方石淋通胶囊中夏佛塔苷的含量[J].中南药学,2013(7):539-541. |
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| 作者姓名： | 马杰 童珂 罗晖明 丁野 胡娟妮 李文莉 |
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| 作者单位： | 湖南省食品药品检验研究院,长沙410001 |
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| 基金项目： | 国家药品标准提高研究项目（编号：2011-619） |
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| 摘 要： | 目的建立高效液相色谱法测定复方石淋通中夏佛塔苷的含量。方法采用Agilent C18色谱柱（250mm×4.6 mm,5μm）,流动相为甲醇-0.5%冰醋酸水溶液（25：75）,流速1.0 mL min-1,检测波长为272nm,柱温30℃。结果夏佛塔苷进样量在0.165 12～4.128μg（r=1,n=7）与峰面积线性关系良好,平均加样回收率（n=6）为100.6%,RSD=1.8%。结论本法灵敏度高,操作简便,准确可靠,可用于复方石淋通胶囊中夏佛塔苷的含量测定。
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| 关 键 词： | 复方石淋通胶囊 夏佛塔苷 高效液相色谱法 |
Determination of schaftoside in Fufang Shilintong capsules by HPLC |
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| MA Jie,TONG Ke,LUO Hui-ming,DING Ye,Hu Juan-ni,LI Wen-li.Determination of schaftoside in Fufang Shilintong capsules by HPLC[J].Central South Pharmacy,2013(7):539-541. |
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| Authors: | MA Jie TONG Ke LUO Hui-ming DING Ye Hu Juan-ni LI Wen-li |
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| Institution: | (Hunan Institute for Food and Drug Control,Changsha 410001) |
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| Abstract: | Objective To establish an HPLC method to determine the content of schaftoside in Fufang Shilintong capsules.Methods The Agilent C18（250 mm×4.6 mm,5 μm） column was used.The mobile phase consisted of methanol-0.5% acetic acid（25 ：75） at 1.0 mL min-1.The detection wavelength was 272 nm and the column temperature was 30 ℃.Results The calibration curve was in good linearity within 0.1651 2-4.128 μg（r = 1,n = 7）.The average recovery was 100.6%,RSD was 1.8%（n = 6）.Conclusion The method is simple and accurate.It can be used for content determination of schaftoside in Fufang Shilintong capsules. |
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| Keywords: | Fufang Shilintong capsule schaftoside HPLC |
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