珠子参总皂苷肠吸收机制研究

目的研究珠子参总皂苷在大鼠的肠吸收动力学特征。方法采用大鼠肠外翻模型,分别收集不同质量浓度珠子参总皂苷给药后不同时间点的肠囊液样品,采用HPLC对人参皂苷Ro、竹节参皂苷Ⅳa进行检测,计算其吸收参数来分析其在肠道的吸收特征。结果不同质量浓度的珠子参总皂苷中人参皂苷Ro、竹节参皂苷Ⅳa在各肠段均为线性吸收,R2均〉0.9,符合一级吸收;人参皂苷Ro和竹节参皂苷Ⅳa回肠累积吸收量和吸收速率高于空肠（P〈0.05）。人参皂苷Ro和竹节参皂苷Ⅳa在空、回肠中的吸收速率常数（Ka）随着剂量增加而增加（P〈0.05）。结论人参皂苷Ro和竹节参皂苷Ⅳa在回肠具有选择吸收性,在空、回肠中为被动转运。

Mechanism of intestinal absorption of total saponin in Panacis Majoris Rhizoma

Objective To study the dynamic absorption of total saponins in Panacis Majoris Rhizoma in the small intestines of rats,and discuss the metabolism.Methods The absorption ingredients of Panacis Majoris Rhizoma were investigated by the model of in vitro everted intestinal sac.The intestinal sac liquors of the jejunum and ileum were collected at different intervals and ginsenoside Ro and chikusetsusaponin Ⅳ a were detected by HPLC as representative markers.The accumulative absorption quantity and the constant of absorption rate（Ka） of ginsenoside Ro and chikusetsusaponin Ⅳ a were calculated.Results At different concentrations of Panacis Majoris Rhizoma,ginsenoside-Ro and chikusetsusaponin Ⅳ a in various intestinal sections showed linear absorption（R2 0.9）,conformed to zero order absorption rate.In the jejunum and ileum the Ka of ginsenoside Ro and chikusetsusaponin Ⅳ a increased with the raised dosage of Panacis Majoris Rhizoma（P 0.05）.Conclusion At low dosage ginsenoside Ro and chikusetsusaponin Ⅳ a in the jejunum is selective,while in the jejunum and ileum ginsenoside Ro and chikusetsusaponin Ⅳ a are passively absorbed.