非小细胞肺癌Survivin和Skp2的表达及其临床意义

目的检测Survivin和Skp2蛋白在非小细胞肺癌中的表达,探讨其相互关系及临床意义。方法用实时定量PCR的方法检测30例非小细胞肺癌组织及其对应的癌旁组织中survivin mRNA的表达,应用免疫组织化学法检测50例非小细胞肺癌组织中Survivin和Skp2的表达,统计Survivin与病理特征及Skp2的关系。结果 76.7%（23/30）肿瘤组织survivin mRNA表达量明显高于对应的癌旁组织。Survivin蛋白在癌组织的阳性表达率（74.0%）显著高于癌旁组织（16.7%）,χ2=25.9,P〈0.001。Skp2在非小细胞肺癌组织中的阳性表达率（70.0%）也显著高于癌旁组织（20.0%）,χ2=18.8,P〈0.001）。Survivin表达与非小细胞肺癌的病理类型、分化程度、肿瘤分期以及是否有淋巴结转移无统计学意义的相关性（P〉0.05）。Survivin表达与Skp2表达呈正相关（χ2=8.32,P〈0.05）。结论 Survivin在肺癌组织中高表达,其阳性表达率与肿瘤病理类型、肿瘤分期、细胞分化程度和淋巴结转移无明显相关性,与Skp2表达呈正相关。

Expression and clinical significance of Survivin and Skp2 in non-small cell lung cancer

Objective To detect the expression of Survivin and Skp2 protein in non-small cell lung cancer, and explore their clinical significance and correlation. Methods survivin mRNA expression in 30 cases of non-small cell lung cancer tissues and paracancerous tissues were detected by real-time fluorescence quantitative PCR. Immunohistochemical Envision method was used to detect the expression of Survivin and Skp2 in 50 cases of non-small cell lung cancer tissues,explored the correlation between Survivin,pathological features and Skp2. Results 76. 7% expression rate of survivin mRNA in tumor tissues was higher than that in their adjacent mucosa. The positive expression rate （ 74. 0% ） of Survivin in tumor tissues was higher than that （ 16. 7% ） in adjacent mucosa tissues. The positive expression rate （ 70. 0% ） of Skp2 in tumor tissues was also higher than that （ 20. 0%） in adjacent mucosa tissues. There was non-statistical correlation between expressive of Survivin and histological type,clinical stage,differentiation and lymph node metastasis（ P 0. 05） . There was positive correlation between expression of Survivin and Skp2（ χ2 = 8. 32,P 0. 05） . Conclusions The expression of Survivin and Skp2 in non-small cell lung cancer is higher than that in adjacent mucosa tissues and not related to histolog- ical type,clinical stage,differentiation and lymph node metastasis. There is positive correlation between expres-sion of Survivin and Skp2.