| E2F3基因真核表达载体的构建和表达 |
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| 引用本文： | 郭新,张跃平,沈维东,何崇生,成兵,陆凤英,周树军.E2F3基因真核表达载体的构建和表达[J].中国现代医学杂志,2007,17(2):160-162. |
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| 作者姓名： | 郭新 张跃平 沈维东 何崇生 成兵 陆凤英 周树军 |
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| 作者单位： | 南通大学附属医院,泌尿外科,江苏,南通,226000 |
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| 基金项目： | 南通大学校科研和教改项目 |
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| 摘 要： | 目的克隆人膀胱肿瘤组织E2F3基因及构建E2F3基因真核表达载体并研究其表达。方法取新鲜膀胱肿瘤组织,提取总RNA,采用逆转录-聚合酶联链反应(RT-PCR)扩增E2F3基因,构建其真核表达载体,测序分析及转染膀胱肿瘤细胞,Western Blot法检测其表达情况。结果成功克隆人膀胱肿瘤组织E2F3基因、构建E2F3基因真核表达载体,转染后成功表达。结论人膀胱肿瘤组织E2F3基因克隆及其真核表达载体构建成功,可用于肿瘤生物学的研究。
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| 关 键 词： | 膀胱肿瘤 E2F3基因 克隆 表达载体 |
| 文章编号： | 1005-8982（2007）02-0160-03 |
| 收稿时间： | 2006-06-20 |
| 修稿时间： | 2006-06-20 |
Construction and expression of eukaryotic expression vector containing gene E2F3 |
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| GUO Xin,ZHANG Yue-ping,SHEN Wei-dong,HE Chong-sheng,CHENG Bing,LU Feng-ying,ZHOU Shu-jun.Construction and expression of eukaryotic expression vector containing gene E2F3[J].China Journal of Modern Medicine,2007,17(2):160-162. |
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| Authors: | GUO Xin ZHANG Yue-ping SHEN Wei-dong HE Chong-sheng CHENG Bing LU Feng-ying ZHOU Shu-jun |
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| Institution: | Department of Urology, the Affiliated Hospital of Nantong University, Nantong, Jiangsu 226000, P.R. China |
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| Abstract: | Objective] To clone human bladder tumor E2F3 gene, construct its eukaryotic expression vector and expression in the BIU-87 cell. Methods] E2F3 gene was obtained from bladder tumor by RT-PCR. Molecular cloning technique was used to construct this kind of eukaryotic expression vector. pEGFP-E2F3 was identified by sequencing. Using liposome-mediated transfection, the eukaryotic expression vector pEGFP-E2F3 was transfected into BIU-87 cell. Results] The eukaryotic expression vector pEGFP-E2F3 was constructed correctly and was expressed in BIU-87 cell. Conclusion] These results suggested that this new kinds of eukaryotic expression vector could serve as a new tool and methods for neoplasm etiology study and gene therapy. |
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| Keywords: | bladder tumor E2F3 gene clone expression vector |
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