人肺癌细胞药物敏感性的琼脂培养MTT测定

采用修改Ｈａｍｂｕｒｇｅｒ－Ｓａｌｍｏｎ体外双层琼脂法与琼脂培养ＭＴＴ法同时对人肺癌细胞进行药物敏感性测定。结果表明：琼脂培养ＭＴＴ法成功率为１００％（４２／４２）；双层琼脂法成功率为５０％（２１／４２）。两法同时可供药敏分析的１２６药物浓度次中，琼脂培养ＭＴＴ法测定肺癌细胞的药物敏感率为３２．５％（４１／１２６）。双层琼脂法的药物敏感度为３９．７％（５０／１２６），两者间无明显差异（Ｐ〉０．０５

CHEMOSENSITIVITY TESTING OF FRESH HUMAN LUNG CANCER CELLS CELLS IN AGAR CULTURE USING THE MTT COLOR IMETRIC ASSAY

In vitro drug sensitivity testing,both of the human tumor colony assay, developed by Hamburger and Salmon, and the MTT assay of agar culture, were performedwith fresh lung cancer samples simultaneously. Date showed that the successful rate of theMTT assay in agar culture was l00 % (42/42) and that of the human tumor colony assaywas only 50 % (21/42). The chemosensitivity rate of the MTT assay in agar culture tohuman lung cancer cells was 32.5 % (41/126) and that of the human tumor colony assaywas 39.7% (50/126). There was no significant difference in sensitivity tested by the twomethods( p>0.05). In comparing the MTT assay in agar culture to the human tumorcolony assay, there was 92.8% (117/126) concordance for sensitivity or resistance. Our preliminary data suggested that the MTT assay in agar culture can replace the humantumor colony assay for predicting drug sensitivity of human tumor clincally.