Effect of haemodialysis on the oxidative stress and antioxidants in diabetes mellitus |
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Authors: | E Dursun B Dursun G Suleymanlar T Ozben |
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Institution: | (1) Department of Biochemistry, Medical Faculty Akdeniz University, Antalya, Turkey;(2) Department of Nephrology, Medical Faculty Akdeniz University, Antalya, Turkey |
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Abstract: | Abstract Oxidative stress has been defined as a loss of counterbalance between free radical or reactive oxygen species (ROS) production
and antioxidant systems. It is involved in the pathogenesis of different chronic diseases. High levels of ROS production via
different biochemical mechanisms accompany diseases like type 2 diabetes mellitus (DM) and end-stage renal disease (ESRD).
Elevated oxidative status and reduced antioxidant defence systems in patients with DM and ESRD accelerate the prevalence of
atherosclerosis and other chronic complications. Our aim was to reveal the effects of diabetes and haemodialysis (HD) separately
and together on oxidative stress. In our study, we included 20 diabetic (DM) patients with no renal disease, 20 non-diabetic
haemodialysis (HD), 20 diabetic haemodialysis (DHD) patients and 20 healthy volunteers. We have determined the levels of lipid
peroxidation expressed as thiobarbituric acid-reactive substances (TBARS), oxidative protein damage as indicated by protein
carbonyl (PCO) content and activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione
peroxidase (GSHPx) in all patient groups and healthy subjects. We found enhanced oxidative stress in all patient groups due
to an increase in lipid peroxidation (TBARS) and increased oxidative protein damage in terms of PCO content and reduced activities
of SOD, CAT and GSH-Px. Oxidative stress was more profound in diabetic patients undergoing haemodialysis. We conclude that
both diabetes and dialysis increase oxidative stress and their combined effect on oxidative stress is the highest in magnitude
as observed in diabetic patients undergoing haemodialysis. |
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Keywords: | Protein oxidation Lipid peroxidation Antioxidant enzymes Diabetes mellitus Haemodialysis |
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