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| 旋转式荧光定量PCR在临床应用中的价值 | |
| 引用本文： | 李洪敏,高华方,张广宇,王思贤,王甦民,孙桂芝,赵智贤,杨楠. 旋转式荧光定量PCR在临床应用中的价值[J]. 医疗卫生装备, 2010, 31(9): 88-89,93 |
| 作者姓名： | 李洪敏 高华方 张广宇 王思贤 王甦民 孙桂芝 赵智贤 杨楠 |
| 作者单位： | 1. 解放军309医院结研所,北京,100091 2. 博奥生物有限公司,北京,100091 3. 北京市结核病控制研究所,北京,100020 4. 北京胸科医院检验科,北京,100092 |
| 摘 要： | 目的：探讨旋转式荧光定量PCR仪在检测临床标本中的应用价值。方法：通过旋转荧光定量-PCR技术鉴定1132株临床分离株,进行结核分枝杆菌的菌种鉴定和定量分析,同时进行抗酸染色和培养法。结果：总阳性检出率为48.64%,其中菌阳检出率为96.56%（253/262）,阴性符合率为77.85%（369/474）,菌种鉴定特异性实验符合率为100%。结论：旋转氏荧光定量PCR仪改进后更加快速、敏感,与其配套使用的试剂特异性强,是在检测结核分枝杆菌复合群的同时,又能进行非结核分枝杆菌鉴定辅助诊断的好方法。 |
| 关 键 词： | 结核分支杆菌 Line-geneK-FQ-PCR 定量分析 菌种鉴定 |
Application of Rotary Fluorescent Quantitative PCR in Clinical Detection of M.tuberculosis |
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| LI Hong-min,GAO Hua-fang,ZHANG Guang-yu,WANG Si-xian,WANG Su-min,SUN Gui-zhi,ZHAO Zhi-xian,YANG Nan. Application of Rotary Fluorescent Quantitative PCR in Clinical Detection of M.tuberculosis[J]. Chinese Medical Equipment Journal, 2010, 31(9): 88-89,93 | |
| Authors: | LI Hong-min GAO Hua-fang ZHANG Guang-yu WANG Si-xian WANG Su-min SUN Gui-zhi ZHAO Zhi-xian YANG Nan |
| Affiliation: | 1.Tuberculosis Research Institute, the 309th Hospital of PLA, Beijing 100091, China; 2.Capital Bio Corporation, Beijing 100091, China; 3.Beijing Research Institute for Tuberculosis Control, Beijing 100020, China; 4.Clinical Laboratory Department, Beijing Chest Hospital, Beijing 100092, China) |
| Abstract: | Objective To discuss the application of rotary fluorescent quantitative PCR instrument in clinical detection of M. tuberculosis. Methods 1 132 clinical isolated strains were tested by rotary fluorescent quantitative PCR（FQ-PCR） for strain identification and quantitative analysis and specimens were examined microscopically with Ziehl -Neelsen staining and cultured at the same time. Results Total positive detection rate was 48.6% and positive detection rate 96.56%（253/262） in positive acid fast stain with negative coincidence 77.85% （369/474）. Coincidence of strain identification was 100% . Conclusion Using rotary FQ-PCR instrument with supporting reagent can effectively identify Mycobacterium tuberculosis complex and diagnose tuberculosis. |
| Keywords: | M. tuberculosis Line-geneK-FQ-PCR quantitative analysis strain identification |
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