体腔积液薄层液基细胞涂片免疫组化染色方法研究

目的 探讨体腔积液薄层液基细胞涂片最佳免疫组化染色方法.方法 收集有组织学对照的恶性胸水68例,每例选12张石蜡切片,作为A组,将每例胸水用薄层液基细胞术制片36张,随机平均分成B、C、D三组;B组经苏木精-伊红（HE）染色及柠檬酸或EDTA修复退色,做免疫组化双重染色,C组经HE染色,用盐酸方法退色,做免疫组化染色,D组不经HE染色,直接做免疫组化.将结果与A组组织病理诊断比较并进行统计学分析.结果 各组别诊断准确率分别为A组100％（68/68）,B组98％（67/68）,C组92％（62/68）,D组90％（61/68）;A组与B组确诊率差异无统计学意义（P＞0.05）,A、B组确诊率高于C组及D组（P＜0.05）.结论 B组方法优于C组和D组,有利于体腔积液中癌细胞鉴别诊断和分类,为临床治疗提供更加可靠的病理诊断.

Research on immunohistochemical stainings for thinprep cytology smear from body cavity effusion

Objective To investigate the best way of immunohistochemical stainings for thinprep cytology smear from body cavity effusion.Methods 68 cases of malignant pleural effusion with histologic control were selected and 12 paraffin sections from each case were selected as group A.36 smears from each body cavity effusion were made by the technology of liquid based thinlayer cytology and divided into group B,C and D.Group B was given hematoxylin-eosin （HE） staining and repair/ discoloration by citric acid or EDTA for double immunohistochemical staining.Group C was given HE staining and repair/discoloration by hydrochloric acid for immunohistochemical staining.Group D was directly given immunohistochemical staining without HE staining.All these results were compared with histopathologic diagnosis from group A and analyzed statistically.Results The diagnostic accuracy rate of group A,B,C and D was 100％ （68/68）,98％ （67/68）,92％ （62/68） and 90％ （61/68） respectively.The diagnostic accuracy rates had no statistical difference between group A and B （P ＞ 0.05）; while the diagnostic accuracy rate of group A and B were both significantly higher than those of group C and D （P ＜ 0.05）.Conclusion The way of double immunohistochemical staining from group B which is better than the ways from group C and D,is beneficial to differential diagnosis and classification for cancer cell from body cavity effusion,and takes more reliable pathologic diagnosis for clinical therapy.