徐长卿提取物对损伤内皮细胞中乳酸脱氢酶、肿瘤坏死因子及白细胞介素-8活性的影响

目的 研究徐长卿提取物对损伤内皮细胞中炎症介质乳酸脱氢酶（LDH）、肿瘤坏死因子（TNF-α）及白细胞介素-8（IL-8）活性的调节作用,探究徐长卿治疗动脉粥样硬化的作用机制.方法 应用补体蛋白纯品于体外在人脐静脉内皮细胞表面组装亚溶破剂量的C5b-9,建立内皮损伤模型,分别建立提取物高、中、低剂量组及空白、模型及标准品对照组,分别用不同浓度的徐长卿提取物及丹皮酚标准品处理细胞,应用紫外分光光度法及ELISA试剂盒测定细胞培养上清液中LDH、TNF-α及IL-8浓度.结果 提取物高剂量组LDH[（119.85±4.12）U/L]及IL-8[（33.65±2.48）ng/L]与模型对照组[（200.49±3.31） U/L,（40.98±0.60）ng/L]比较,差异有高度统计学意义（P＜0.01）;高剂量[（94.75±3.19）ng/L]及中剂量组[（92.81±1.78）ng/L]与模型对照组[（108.00±2.30）ng/L]比较,均可降低TNF-α水平（P＜ 0.01）.结论 徐长卿可能通过降低炎症介质LDH、TNF-α及IL-8活性,从而修复内皮细胞损伤来达到治疗动脉粥样硬化的目的.

The infuence of Cynanchum Extract on lactate dehydrogenase, tumor necrosis factor and interleukin-8 activity in endothelial injury cells

Objective To research the regulation of Cynanchum Extract for inflammatory mediators LDH,TNF-α and IL-8 in endothelial cells,and explore the mechanism of Cynanchum treatment of atherosclerosis.Methods The sublysis dose of C5b-9 was assembled on human umbilical vein endothelial cell surface in vitro by pure complement proteins,and endothelial injury model was established.Extract of high,middle and low doses,model and standard groups were built; cells were cultured in different concentration drugs.Then the concentration of LDH,TNF-α and IL-8 in cell culture supernatant was measured by UV and ELISA kits.Results LDH and IL-8 activity reduced in extract high-dose group [（119.85±4.12） U/L] and IL-8 [（33.65±2.48） ng/L] significantly,compared with model control group [（200.49±3.31） U/L,（40.98±0.60） ng/L] had statistically significant difference （P ＜ 0.01）.TNF-α level reduced in high-dose [（94.75±3.19） ng/L] and medium-dose groups [（92.81± 1.78） ng/L],compared with model control group [（108.00±2.30） ng/L] had statistically significant difference （P ＜ 0.01）.Conclusion Cynanchum may repair endothelial injury cells for therapy atherosclerosis by reducing the activity of inflammatory mediators LDH,TNF-α and IL-8.