Murine immunoglobin response to sterile talc injection

Mice were injected subcutaneously with sterile talc, and the changes in serum IgG, IgM, and IgA were investigated at various time intervals. Three talc-injection schedules were used: a single injection of talc; a double injection of talc; and two injections of talc at different times. The results showed that: (1) a single injection of talc resulted in an increase in serum IgM at 24 hr, and an increase in IgG at 24–48 hr; (2) a double injection of talc did not result in a significant difference in immunoglobulin levels from those observed after a single injection; (3) two injections of talc given at different times did not result in a response greater than that observed following a single injection of talc. No changes from control values were observed in IgA. No evidence for the presence of an antigen was found.