| 6His-hEra融合蛋白的高效表达 |
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| 引用本文： | 纪宗玲,陈苏民,刘继中,李毅,吴元明,张晓楠.6His-hEra融合蛋白的高效表达[J].中国免疫学杂志,2002,18(11):752-754. |
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| 作者姓名： | 纪宗玲 陈苏民 刘继中 李毅 吴元明 张晓楠 |
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| 作者单位： | 1. 第四军医大学生物化学与分子生物学教研室,西安,710032
2. 西京医院骨科研究所 |
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| 基金项目： | 国家自然科学基金资助 (No3 0 0 0 0 0 91，3 9870 3 80 ) |
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| 摘 要： | 目的 :在原核表达系统中对人Era基因进行表达。方法 :人era编码区基因克隆入pUC19质粒中 ,经全自动序列分析仪测序正确后 ,再克隆入表达载体pRSETB中 ,构建成融合 6个组氨酸的高效表达载体pRSETB Era ,转化大肠杆菌BL2 1DE3(plysS)诱导表达。结果 :经IPTG诱导后 4h ,SDS PAGE及凝胶密度扫描分析 ,表达出分子量约 4 1kD的蛋白 ,占菌体总蛋白的 5 1 2 %。结论 :该基因在大肠杆菌中的高效表达为人era的功能研究打下了基础。
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| 关 键 词： | 人Era GTP结合蛋白 表达 测序 |
| 文章编号： | 1000-484X(2002)11-0752-03 |
High level expression of 6His-hEra |
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| JI Zong Ling,CHEN Su Min,LIU Ji Zhong et al.High level expression of 6His-hEra[J].Chinese Journal of Immunology,2002,18(11):752-754. |
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| Authors: | JI Zong Ling CHEN Su Min LIU Ji Zhong |
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| Institution: | JI Zong Ling,CHEN Su Min,LIU Ji Zhong et al.Department of Biochemistry and Molecular Biology,Fourth Military Medical University,Xi'an 710032 |
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| Abstract: | Objective:To study the expression of human Era.Methods:The encoding region of human Era gene was inserted into pUC19 plasmid and sequenced,the gene fragment was then cloned into the prokaryotic fusion expression vector pRSET B to contruct recombinant expression plasmid pRSET B hEra.The recombinant protein was expressed in E.coli BL21DE3(plys S).Results:6His hEra was expressed after induced by IPTG and took 51.2% of the total cell lysate and mainly existed in the inclusion body.Conclusion:High level of human Era was established and this result may shed light on the further study on function of human Era. |
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| Keywords: | Human Era GTP binding protein Expression Sequence |
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