| 环氧化酶Ⅱ抑制剂体外筛选模型的研究 |
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| 引用本文： | 汪志军,谢晨,张娟,孙进,周建伟,王旻,张玉彬. 环氧化酶Ⅱ抑制剂体外筛选模型的研究[J]. 中国药学杂志, 2003, 38(4): 266-268 |
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| 作者姓名： | 汪志军 谢晨 张娟 孙进 周建伟 王旻 张玉彬 |
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| 作者单位： | 1. 中国药科大学分子生物学实验室,江苏,南京,210009
2. 南京医科大学毒理学实验室,江苏,南京,210029 |
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| 基金项目： | 南京医科大学应用毒理学重点实验室开放基金 (K2 0 34 )，江苏省自然科学基金 (BK990 99) |
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| 摘 要： | 目的建立环氧化酶Ⅱ活性的检测模型，提供环氧化酶Ⅱ抑制剂的筛选模型及非甾体类抗炎药(nonsteoroidal anti inflammatory drug,NSAID)筛选的方法。方法利用NIH 3T3细胞在血清存在情况下能诱导生成环氧化酶Ⅱ的特性，通过加入底物花生四烯酸，检测终产物前列腺素E-2(PGE-2)含量的变化，判断化合物对环氧化酶Ⅱ的作用。以rofecoxib(罗非昔布)为阳性药来判断该模型能否用于环氧化酶Ⅱ活性的检测。结果Rofecoxib能明显的抑制环氧化酶Ⅱ的活性。结论该方法可以用于环氧化酶Ⅱ抑制剂的筛选。
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| 关 键 词： | 环氧化酶 PGE2 NIH3T3 筛选模型 |
| 文章编号： | 1001-2494(2003)04-0266-03 |
| 收稿时间： | 2002-04-02; |
| 修稿时间： | 2002-04-02 |
Study on screening the cyclooxygenase-2 inhibitor by in vitro method |
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| WANG Zhi-jun,XIE Chen,ZHANG Juan,SUN Jin,ZHOU Jian-wei,WANG Min,ZHANG Yu-bin. Study on screening the cyclooxygenase-2 inhibitor by in vitro method[J]. Chinese Pharmaceutical Journal, 2003, 38(4): 266-268 |
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| Authors: | WANG Zhi-jun XIE Chen ZHANG Juan SUN Jin ZHOU Jian-wei WANG Min ZHANG Yu-bin |
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| Affiliation: | 1.The Laboratory of Molecular Biology,China Pharmaceutical University，Nanjing 210009,China；2.The Department of Toxicology,Nanjing Medical University，Nanjing 210029,China |
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| Abstract: | OBJECTIVE To establish the assay model of cyclooxygenase-2 activity and provide the screening method of NSAIDs.METHODS Observations were made that cyclooxygenase-2 was induced in the exist of FCS.The inhibitory potency of the compounds was evaluated by determining the concentration of PGE 2.Rofecoxib employed as the reference drug was used to evaluate whether this method was available to determine the inhibitory potency of cyclooxygenase-2.RESULTS The results proved that rofecoxib can inhibit the activity of cyclooxygenase-2.CONCLUSION The in vitro method can be used to screen the cyclooxygenase-2 inhibitor. |
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| Keywords: | cyclooxygenase 2 PGE 2 NIH 3T3 screen model |
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