| 时间分辨荧光各向异性及其在生物大分子研究中的应用 |
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| 引用本文： | 吴道澄,万明习. 时间分辨荧光各向异性及其在生物大分子研究中的应用[J]. 生物医学工程学杂志, 2002, 19(4): 712-714 |
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| 作者姓名： | 吴道澄 万明习 |
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| 作者单位： | 西安交通大学生物医学工程系,西安,710049 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 170 2 71) |
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| 摘 要： | 概述了时间分辨荧光各向异性（TRAMS）的工作原理和发展概况，描述了常用荧光探针，标记物及大分子的一般标记方法，讨论了其测定方法，研究特点及生物大分子中的构象及结构变化等研究方面的应用，展望了未来的应用前景。
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| 关 键 词： | 生物大分子 时间分辨荧光各向异性 荧光探针 荧光偏振现象 光物理 荧光标记 |
Time-Resolved Fluorescence Anisotropy Measurement and Its Application in the Research of biomacromolecule |
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| Wu Daocheng Wan Mingxi. Time-Resolved Fluorescence Anisotropy Measurement and Its Application in the Research of biomacromolecule[J]. Journal of biomedical engineering, 2002, 19(4): 712-714 |
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| Authors: | Wu Daocheng Wan Mingxi |
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| Affiliation: | Department of Biomedical Engineering, Xi'an Jiaotong University, Xi'an 710049. |
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| Abstract: | In this symposium, we reviewed the principle and development of time-resolved fluorescence anisotropy measurement. Its method of measurement, characteristics and applications in the research of biomacromolecule, configuration and molecular structure have been discussed. Its potential applications are also illustrated. |
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| Keywords: | Time-Resolved fluorescence anisotropy measurement Biomacromolecule Fluorescence probe |
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