典型与不典型免疫表型慢性淋巴细胞白血病的预后相关因素分析 |
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引用本文: | 曹鑫,徐卫,吴雨洁,乔纯,刘琼,范磊,缪扣荣,李建勇.典型与不典型免疫表型慢性淋巴细胞白血病的预后相关因素分析[J].中华血液学杂志,2006,30(1):450-453. |
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作者姓名: | 曹鑫 徐卫 吴雨洁 乔纯 刘琼 范磊 缪扣荣 李建勇 |
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作者单位: | 南京医科人学第一附属医院、江苏省人民医院血液科,210029; |
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基金项目: | 江苏省医学领军人才基金江苏省医学重点人才基金江苏省自然科学基金江苏省社会发展计划基金国家自然科学基金 |
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摘 要: | Objective To analyze the proguostic factors for chronic lymphocytic leukemia (CLL) with typical and atypical immunophenotype. The parameters analyzed included sex, age, Binet stages, abso-lute lymphocyte count (ALC), immunoglobulin heavy-chain variable region (IgVH) gene mutation status, ZAP-70 protein, CD38 expression and cytogenetic aberrations. Methods According to the clinical guideline and scoring system for CLL in Britain, among 77 patients, 61 patients with score 5 called typical immunophe-notype CLL, 16 with score 4 or 3 were atypical immunophenotype CI,L. Multiparameter flow cytometry was employed for immunophenotypic analysis in 77 CLL patients for CD5, CD19, CD23, FMC7, slg, CD20, CD79h expression and ZAP-70 protein and CD38. IgVH mutation status was detected by multiplex RT-PCR and sequencing of the purified PCR amplification products. Fluorescence in situ hybridization (FISH) and a panel of probes were used to detect cytogenetic aberrations. Results There was no significant difference be-tween the two groups in sex, age, ZAP-70 and IgVH mutation status (P =0.398, P =0. 189, P =0.268 and P =0. 131, respectively). The incidence of ALC≥50 × 109/L, Binet B + C, CD38 ≥30% in atypical CLL patients(43.8%, 87.5% and 43.8%, respectively) were higher than that in typical group (16.4%, 36.1% and 16.4%, respectively) (P = 0. 026, P < 0. 01 and P = 0. 026, respectively). The proportion of typical patients (26. 8%) with a 13q14 deletion as sole abnormality was higher than that of atypical patients (7.6%), and that with deletion of 11q22 or 17p13 was lower than that of atypical patients (12.2% vs 46.2%) (P = 0. 022). Conclusion There were obvious differences between the typical immunophenotype CLL and atypical CLL in ALC, Binet stages, CD38 expression level and cytogenetic aberrations.
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关 键 词: | 白血病 淋巴细胞 慢性 免疫表型 预后 |
Prognostic factors for chronic lymphocytic leukemia with typical and atypical immunophenotype |
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Abstract: | |
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Keywords: | Leukemia lymphocytic chronicImmunophenotypePrognosis |
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