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| HI_(47),一种抗人成熟B细胞单克隆抗体制备、鉴定及其生物学特性研究 | |
| 引用本文： | 杨希峰,沈德诚,金宇光,白金芬,汤美华,张金香,卞钟元,陈璋. HI_(47),一种抗人成熟B细胞单克隆抗体制备、鉴定及其生物学特性研究[J]. 现代免疫学, 1990, 0(2) |
| 作者姓名： | 杨希峰 沈德诚 金宇光 白金芬 汤美华 张金香 卞钟元 陈璋 |
| 作者单位： | 中国医学科学院血液学研究所免疫室 天津(杨希峰，沈德诚，金宇光，白金芬，汤美华，张金香，卞钟元)，中国医学科学院血液学研究所免疫室 天津(陈璋) |
| 摘 要： | 用人扁桃腺细胞免疫BALB/c 小鼠的脾细胞与小鼠NS_1骨髓癌细胞融合,得到一种单克隆抗体HI_(47)(IgG_3),能够结合家兔补体,主要与分化后期的人B细胞反应,还可与巨噬细胞、树突状细胞、内皮细胞及部分激活T细胞结合。HI_(47)识别抗原的分子量为37/35KD,其分布与CD_(20)相似。第四届国际人类白细胞分化抗原会议将HI_(47)命名为CD_(20)+X。本文还初步探讨了HI_(47)抗体抑制SAC诱导B细胞活化增殖的作用。 |
PREPARATION AND CHARACTERIZATION OF A MONOCLONAL ANTIBODY HI47 DIRECT TO HUMAN B LYMPHOCYTE |
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| Abstract: | A monoclonal antibody H147 (lgG3) is obtained in our laboratory by cell fusion De-tween NSl murine myeloma cells and BALB/c murine spleen cells immunized with human tonsil cells. It is able to activate rabbit complement. HI47 is reactive with more mature B lymphocytes, dendritic cells, endothelial cells, macrophages and a subset of activated T lymphocytes. The molecular weight of HI47 antigen is 37/35KD. The distribution of this antigen is correlative to that of CD20. It has been characterized as CD20 + X in the 4th International Conference on Human Leukocyte Differentiation Antigen. Inhibition of proliferative response of B lymphocyte to SAC by HI47 is also observed. |
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