| 沙棘汁中总黄酮提取及纯化研究 |
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| 引用本文： | 杨云裳,张应鹏,李春雷,许建国,南文杰. 沙棘汁中总黄酮提取及纯化研究[J]. 时珍国医国药, 2011, 22(3): 570-572. DOI: 10.3969/j.issn.1008-0805.2011.03.022 |
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| 作者姓名： | 杨云裳 张应鹏 李春雷 许建国 南文杰 |
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| 作者单位： | 兰州理工大学石油化工学院,有机与药物化学研究所,甘肃,兰州,730050 |
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| 基金项目： | 甘肃省自然科学基金，兰州理工大学校基金 |
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| 摘 要： | 目的建立沙棘汁总黄酮的提取及纯化工艺。方法溶剂提取法提取黄酮,大孔吸附树脂纯化,利用紫外分光光度计检测黄酮的含量。结果在50℃的条件下用V(醋酸乙酯):V(95%乙醇)=3:7回流提取3次,每次2 h,料液比为1:10(W/V),并用大孔吸附树脂纯化。在此条件下,黄酮类化合物的得率达1.611%,纯度为20.18%。结论上述方法可以有效提取及纯化沙棘汁中的总黄酮。
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| 关 键 词： | 提取 纯化 大孔吸附树脂 沙棘汁 总黄酮 |
Extraction and Purification of Total Flavones from Fruit Juice of Hippophae rhamnoides L. |
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| YANG Yun-shang,ZHANG Ying-peng,LI Chun-lei,XU Jian-guo,NAN Wen-jie. Extraction and Purification of Total Flavones from Fruit Juice of Hippophae rhamnoides L.[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(3): 570-572. DOI: 10.3969/j.issn.1008-0805.2011.03.022 |
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| Authors: | YANG Yun-shang ZHANG Ying-peng LI Chun-lei XU Jian-guo NAN Wen-jie |
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| Affiliation: | (Institute of Organic & Medicinal Chemistry,School of Petrochemical Engineering,Lanzhou University of Technology,Lanzhou 730050,China) |
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| Abstract: | Objective To study the extraction and purification of total flavone from fruit juice of Hippophae rhamnoides L. Methods Solvent-extraction process was used for flavones extraction,macroporous resin was used to purify the total flavones and UV spectrophotometry was used to analyze the contents of total flavones. Results The optimum conditions were 50℃,1:10(W/V),the mixture extracting solvent ratio of Vethyl acetate to V95%ethanolwere 3:7,extracting 3 times and 2h for each time,purification of total flavones by macroporous resin.The extraction yeild of flavones was 1.611%,and its purity was 20.18%. Conclusion The total flavones from fruit juice of Hippophae rhamnoides L.can be effectively extracted and purified by above-mentioned methods. |
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| Keywords: | Extraction Purification Macroporous resin Fruits of Hippophae rhamnoides L Total flavones |
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