水杨酸对丹参幼苗营养期生长及品质影响的研究

目的以丹参幼苗为材料,研究水杨酸对丹参种苗根部丹参酮类、丹酚酸类成分含量、抗氧化酶活性以及根部生长性状的影响。方法将丹参种苗进行根部灌溉水杨酸处理,按照生测法和HPLC法定期测定生长指标及丹参酮ⅡA、隐丹参酮和丹酚酸B的含量,并进行了超氧化物歧化酶(SOD)活性的测定。结果经水杨酸处理后,丹参根的丹参酮ⅡA、隐丹参酮、丹酚酸B含量均高于对照,SOD酶活性增强,在水杨酸0.1～3 mmol/L的范围内,随浓度的升高和处理时间的延续而含量增加。高浓度(5 mmol/L)处理下对根部生长有一定的抑制作用,根部的丹参酮ⅡA、隐丹参酮、丹酚酸B含量也较最佳处理浓度(3 mmol/L)的效应有所减弱。结论在一定的浓度范围下,浇灌水杨酸能促进丹参根部丹参酮ⅡA、隐丹参酮、丹酚酸B含量的积累,并能增加SOD酶活性,以及丹参根部的根长、根直径及根鲜重。

Effects of Salicylic Acid on the Contents of Tanshinones and Salvianolic Acids in the Roots of Salvia miltiorrhiza

Objective To study the effects of SA on the contents of tanshinones and Salvianolic acid,the activity of anti-oxidative enzyme and the root properties of Salvia miltiorrhiza.Methods After treatment with SA,the growth index and TanshinoneⅡA,cryptotanshinone、salvianolic acid B contents in the roots were assayed by the methods of biological determination and HPLC,and the activity of SOD was assayed.Results TanshinoneⅡA,cryptotanshinone,salvianolic acid B contents in the roots were higher than those of control group,Within the concentration of 0.1～3 mmol/L,the contents of TanshinoneⅡA、cryptotanshinone and salvianolic acid B and the acoivity of SOD were gradually increased with the rise of SA concentration and treating time,but under the higher concentration(5 mmol/L),the growth of roots and TanshinoneⅡA、cryptotanshinone and salvianolic acid B contents were decreased.Conclusion Within a certain SA concentration range,SA showed a promotion effect on the root length,width,biomass and TanshinoneⅡA,cryptotanshinone and salvianolic acid B contents.