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| 蛤蚧及其伪品微型DNA条形码的引物筛选 | |
| 引用本文： | 李力,顾海丰,夏云,徐永莉,彭锐,莫邦辉,曾晓茂.蛤蚧及其伪品微型DNA条形码的引物筛选[J].时珍国医国药,2011,22(1):202-205. |
| 作者姓名： | 李力 顾海丰 夏云 徐永莉 彭锐 莫邦辉 曾晓茂 |
| 作者单位： | 1. 广西药用植物园,广西,南宁,530023 2. 中国科学院成都生物研究所,四川,成都,610041;中国科学院研究生院,北京,100049 3. 中国科学院成都生物研究所,四川,成都,610041 4. 中国科学院成都计算机应用研究所,四川,成都,610041 |
| 基金项目： | 国家自然科学基金，国家"十一五"科技支撑项目，留学人员科技活动项目择优资助经费课题 |
| 摘 要： | 文章旨在筛选出适合于扩增蛤蚧及伪品微型DNA条形码引物。通过引物设计软件,经过多序列比对,设计了两对引物mini-barcode F2,mini-barcode R2;mini-barcode F3,mini-barcode R3,并用这两对引物和目前已报道的微型条形码引物PCR扩增蛤蚧及伪品61个样本,结果表明mini-barcode F2,mini-barcode R2和mini-barcode F3,mini-barcode R3这两对引物的PCR扩增成功率分别为100%和90%,而目前已报道的微型条形码引物PCR扩增蛤蚧及其伪品得到了长度约500 bp非微型条形码序列片段。因此目前已报道的微型条形码引物不适用于扩增蛤蚧及伪品,该研究筛选出了适合于蛤蚧及伪品的微型条形码引物。 |
| 关 键 词： | PCR引物 微型条形码 微型条形码引物 蛤蚧及其伪品 |
A Kind of Mini - barcode Primer for Gecko and its Adulterant |
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| LI Li,GU Hai-feng,XIA Yun,XU Yong-li,PENG Rui,MO Bang-hui,ZENG Xiao-mao.A Kind of Mini - barcode Primer for Gecko and its Adulterant[J].Lishizhen Medicine and Materia Medica Research,2011,22(1):202-205. | |
| Authors: | LI Li GU Hai-feng XIA Yun XU Yong-li PENG Rui MO Bang-hui ZENG Xiao-mao |
| Institution: | 1.Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China;2.Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;3.Graduate School of the Chinese Academy of Sciences,Beijing 100049;4.Chengdu Institute of Computer Applications,Chinese Academy of Sciences,Chengdu 610041,China)) |
| Abstract: | To design mini-barcode primers for amplifying gecko and its adulterants.By aligning COI sequences with primer design computer software,we gained two pair primers(mini-barcode F2,mini-barcode R2;mini-barcode F3,mini-barcode R3),and then amplified 61 samples from gecko and its adulterant with three pair primers,including these two pair primers and currently generally used primer.The results showed that the primers(mini-barcode F2,mini-barcode R2) had 100% PCR amplification success rate,the primers(mini-barcode F3,mini-barcode R3) gained 90% PCR amplification success rate,but currently universal primer performed disappointment,we got about 500 bp fragment in PCR amplification with this primers.This fragment is not target fragment.So the currently used primer is not applicable for amplifying gecko and its adulterants,the primers we designed is effective for amplifying gecko and its adulterants. |
| Keywords: | PCR primers mini-barcode Mini-barcode primers Gecko and its adulterants |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |