脉冲光激发系统法检测辐照中药材方法的建立

目的 建立辐照中药材的系统检测方法，为评价中药材质量及安全用药提供参考。方法 利用脉冲光激发光系统（PSL）研究不同来源不同品种中药材在一系列辐照剂量（1、2、4、6、8、10 kGy）辐射后光子计数率的变化规律。以光子计数率700为筛查阈值，对中药材样品进行辐照初筛；以1 kGy作为校正剂量对光子计数率高于700的样品进行校正PSL。结果 选定的28批中药材样品经上述一系列辐照剂量辐射后，光子计数率均比辐照前大幅增加；连续测定同一份样品4次，与第1次相比，光子计数率均呈现40%±10%、60%±10%和70%±10%的下降规律。辐照初筛的403批中药材样品中，光子计数率低于700的有307批，占76.2%；光子计数率高于700的有96批，占23.8%，其中经校正PSL法检测后依法判定为辐照过的阳性样品1批，占全部样品的0.25%。结论 本法简单可靠，准确度较高，可用于辐照中药材的系统筛查。

Establishment of testing method for irradiation of traditional Chinese medicinal materials using PSL system

Objective To establish a pulsed photo stimulated luminescence (PSL) system testing method for irradiation of traditional Chinese medicinal materials (TCMM) and to provide the basis for quality evaluation and safely use of TCMM. Methods PSL system was used to research the photon count rate (PCR) principle of different kinds of TCMM from various habitats after irradiation by 1, 2, 4, 6, 8, and 10 kGy. PCR 700 was chosen as the threshold to previously classify all the samples. And 1 kGy was used to calibrate the samples with PCR over 700 using PSL. Results After a series of irradiation, the PCR of the 28 batches of TCMM all substantially increased. While testing the same sample four times continuously, the PCR comparing to the first time decreased by 40% ± 10%, 60% ± 10%, and 70% ± 10%, respectively. Among 403 batches of TCMM, PCR of 307 batches of samples were below 700 (76.2%), while 96 batches of samples were above 700 (23.8%), and calibrated PSL confirmed one irradiated TCMM sample (0.25%) finally. Conclusion This method is simple, reliable, and accurate, and could be used for system screening of irradiated TCMM.