浙江西南野生金银花种质资源的特性研究

目的 研究浙江西南地区野生金银花种质资源的形态性状、有效成分及遗传特性，为金银花种质资源的开发利用及遗传育种提供依据。方法 采用形态性状调查、HPLC检测及ITS序列分析揭示野生金银花种质的特性。结果 浙江西南地区的6个野生金银花种质在形态性状上差异较小，通过ITS序列分析表明它们之间亲缘关系非常相近，能够与忍冬属其他植物明显区分。6个野生种质与栽培品种之间形态差异和有效成分量差异较大，其中有性状优良的野生种质，如产自浙江省景宁县（ZJJN1）与浙江省青田县（ZJQT）的样品，其绿原酸量分别为1.94%和2.42%，达到《中国药典》2010年版标准。结论 研究野生金银花种质的优良特性，对浙江西南地区野生金银花资源的开发及优良栽培品种的选育具有重要的现实意义。

Characteristics of wild Lonicera Japonica Flos germplasm resources in Southwest Zhejiang Province

Objective Morphological, chemical, and genetic characteristics of wild Lonicera Japonica Flos (LJF) germplasm resources in Southwest Zhejiang Province were discussed for the utilization and breeding of LJF germplasm resources. Methods Morphological investigation, HPLC detection, and ITS analysis were combined to reveal the feature of wild LJF germplasm. Results Few differences in morphology were found among six wild LJF germplasm in Southwest Zhejiang Province and ITS analysis showed the similar result, indicating a closed relationship which was easy to distinguish from the other species in Lonicera Linn. Diversities in morphology and components were found between wild and cultivated LJF. Some wild germplasm, like ZJJN1 and ZJQT, presented good properties, with the contents of chlorogenic acid reaching 1.94% and 2.42%, respectively, which met the requirements of Chinese Pharmacopoeia 2010. Conclusion The investigation on the good characteristics of wild LJF germplasm could provide the practical reference for the development of wild LJF in Southwest Zhejiang Province and breeding of superior cultivars.