| 正交实验优选金花茶种子总多酚的最佳提取工艺 |
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| 引用本文： | 唐前,罗燕英,唐玲,黄连冬,于大永,冯宝民,王永奇.正交实验优选金花茶种子总多酚的最佳提取工艺[J].时珍国医国药,2010,21(4). |
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| 作者姓名： | 唐前 罗燕英 唐玲 黄连冬 于大永 冯宝民 王永奇 |
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| 作者单位： | 1. 大连大学药物研究所,辽宁,大连,116622
2. 广西南宁市金花茶公园,广西,南宁530022 |
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| 摘 要： | 目的 优选金花茶种子中总多酚的提取工艺.方法 采用丙酮-水法从金花茶种子中提取总多酚,利用正交实验优选总多酚最佳提取工艺,考察丙酮浓度、时间、温度、提取次数及料液比对总多酚收率的影响.结果 总多酚的最佳提取工艺为:25倍量的50%丙酮-水,80℃,提取3次,4 h/次,总多酚含量为9.59%,总多酚的收率为1.71%.结论 丙酮-水提取法可用于金花茶种子中总多酚的提取,其中提取溶剂倍量和提取温度对总多酚提取的影响较大.
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| 关 键 词： | 金花茶种子 总多酚 提取工艺 正交实验 |
Optimum Technology for Extraction of Total Polyphenol from Theopsis Chrysantha Hu.Seeds by Orthogonal Test |
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| TANG Qian,LUO Yan-ying,TANG Ling,HUANG Lian-dong,YU Da-yong,FENG Bao-min,WANG Yong-qi.Optimum Technology for Extraction of Total Polyphenol from Theopsis Chrysantha Hu.Seeds by Orthogonal Test[J].Lishizhen Medicine and Materia Medica Research,2010,21(4). |
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| Authors: | TANG Qian LUO Yan-ying TANG Ling HUANG Lian-dong YU Da-yong FENG Bao-min WANG Yong-qi |
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| Institution: | TANG Qian1,LUO Yan-ying2,TANG Ling1,HUANG Lian-dong2,YU Da-yong1,FENG Bao-min1,WANG Yong-qi1 (1.Institute of Materia Medica of Dalian University,Dalian,Liaoning 116622,China,2.Park of Jin Hua Cha of Nanning,Nanning,Guangxi 530022,China) |
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| Abstract: | Objective To optimize the technology for extraction of total polyphenol from Theopsis chrysantha Hu.Seeds.Methods The seeds was extracted using acetone-aqueous,and the extract rate of total polyphenol was studied by orthogonal test,and the effects of solvent concentration of extraction,time of extraction,temperature of extraction,number times of extraction,solvent times of extraction were investigated.Results The optimum extraction technology was as follows: 25 times 50% acetone-aqueous,extracting 3 times a... |
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| Keywords: | Theopsis chrysantha Hu seeds Total polyphenol Extracting technology Orthogonal test |
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