| 二阶导数紫外分光光度法测定酒石酸美托洛尔体外经皮渗透量 |
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| 引用本文： | 王文刚,恽榴红,王睿,周筱青. 二阶导数紫外分光光度法测定酒石酸美托洛尔体外经皮渗透量[J]. 中国药房, 2005, 16(18): 1379-1381 |
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| 作者姓名： | 王文刚 恽榴红 王睿 周筱青 |
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| 作者单位： | 1. 军事医学科学院毒物药物研究所,北京市,100850
2. 解放军总医院临床药理研究室,北京市,100853 |
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| 摘 要： | 目的:建立以二阶导数紫外分光光度法测定酒石酸美托洛尔(MP)体外经皮渗透量的方法。方法:对MP的二阶导数紫外扫描图谱在224nm和234nm波长处出现的特征谷和特征峰间的振幅ΔA进行定量。结果:MP检测浓度在5~60μg/ml范围内线性关系良好,平均回收率为98.89%,RSD平均值为2.05%。结论:本法可排除皮肤渗出物干扰并准确测定MP体外经皮渗透量,且操作简便、快速,结果准确、可靠。
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| 关 键 词： | 二阶导数紫外分光光度法 酒石酸美托洛尔 经皮渗透 |
| 文章编号： | 1001-0408（2005）18-1379-03 |
| 收稿时间： | 2005-02-01 |
| 修稿时间： | 2005-05-31 |
Determination of Transdermal Osmolality of Metoprolol Tartrate by Second-order Derivative UV Spectrophotometry in Vitro |
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| WANG Wengang,YUN Liuhong,WANG Rui,ZHOU Xiaoqing. Determination of Transdermal Osmolality of Metoprolol Tartrate by Second-order Derivative UV Spectrophotometry in Vitro[J]. China Pharmacy, 2005, 16(18): 1379-1381 |
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| Authors: | WANG Wengang YUN Liuhong WANG Rui ZHOU Xiaoqing |
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| Affiliation: | 1.Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China; 2.Dept. of Clinical Pharmacology of PLA General Hospital, Beijing 100853, China |
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| Abstract: | OBJECTIVE:To establish the Second-order Derivative UV-spectrophotometry for the determination of transdermal osmolality of metoprolol tartrate (MP)in vitro.METHODS:The quantitation of the vibration amplitude (ΔA)between the characteristic valley and the characteristic peak of MP appeared at 224nm and 234nm in UV-scanning.RESULTS:The linear concentration range of metoprolol tartrate was 5～60μg/ml,the average recovery was 98.89%with average RSD at 2.05%.CONCLUSION:This method can accurately determine the transdermal osmolality of MP in vitro yet without the interference of skin exudates,its operation is simple and fast and the results are accurate and reliable. |
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| Keywords: | Second-order derivative UV-spectrophotometry Metoprolol tartrate Transdermal permeation |
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