抑郁症患者认知功能障碍与脑源性神经营养因子基因多态性的关系

目的 探讨脑源性神经营养因子（BDNF）基因与抑郁症患者认知功能障碍的关系.方法 采用北京版蒙特利尔认知评估量表（MoCA-BJ）分别对73例抑郁症患者和66例正常人进行认知功能评定,根据MoCA-BJ得分,将抑郁症患者划分为伴认知功能障碍组（36例）和不伴认知功能障碍组（37例）;采用PCR-RFLP技术,检测BDNF基因rs6265及rs12273539两个SNPs位点的基因型.结果 3组之间rs6265位点基因型分布（x2=5.18,P=0.27）、A等位基因携带频率（x2=4.28,P=0.12）、G等位基因携带频率（x2=1.95,P=0.38）差异无统计学意义;不伴认知功能障碍组与健康对照组在rs12273539位点基因型分布,等位基因携带频率上差异无统计学意义（P＞0.05）;而伴认知功能障碍组与健康对照组的rs12273539位点基因型分布差异有统计学意义（x2=8.38,P=0.02）,C等位基因携带频率显著高于对照组（x2=5.40,P=0.02）,T等位基因携带频率低于对照组（x2=6.06,P=0.01）;注意功能在CC、CT、TT三种基因型间的分值差异具有统计学意义（P＜0.01）.结论 BDNF rs12273539（T/C）基因型与抑郁症患者认知障碍相关,伴认知功能障碍的抑郁症患者比健康对照组携带更多的C等位基因;CC基因型的抑郁症患者注意功能的损害更为严重.

Associations between brain-derived neurotrophic factor gene polymorphisms and cognitive disorder in depression

Objective To explore the relationship between polymorphisms of brain-derived neurotrophic factor（BDNF） gene （rs6265 and rs12273539） and cognitive impairment in depressive disorder.Methods All participants including 73 depressed patients and 71 healthy controls were received clinical and cognitive assessments at admission,and then the depression group was divided into two groups by the score of Beijing version of the Montreal Cognitive Assessment （MoCA-BJ）.One was depression with cognitive dysfunction group,and the other was depression without cognitive dysfunction group,with 36 and 37 cases respectively.The polymorphisms of BDNF gene was identified by PCR-RFLP.Results No significant difference for rs6265 gene types（x2=5.18,P=0.27）,A allele carries （x2 =4.28,P=0.12） and G allele carries （x2 =1.95,P=0.38） among the three groups.There was no significant difference for rs12273539 gene types,allele carries between patients without cognitive dysfunction and controls groups（P〉0.05）.There was much more C-allele carries （x2=5.40,P=0.02）and less T-allele（x2=6.06,P=0.01） in patients with cognitive disorder than those in health and it was different in rs12273539 gene types between the two groups（x2=8.38,P=0.02）.CC/CT/CT gene type performed different on attention function （P〈0.01）.Conclusion BDNF rsl2273539（T/C） gene type has relationship with the onset of cognitive disorder in depressed patients,and there are more C-allele carries in depressive patients.The depression patients with CC gene type are worse on the attention function impairement.