精神分裂症患者血浆及单核细胞中microRNA表达水平差异的研究

目的 探讨精神分裂症患者血浆和外周血单核细胞内microRNA（miRNA或miR）表达水平的差异.方法 对连续入组60例精神分裂症患者和72例正常对照,使用实时荧光定量PCR技术检测病例组和对照组血浆和外周血单核细胞内9种miRNA（miR-30e、miR-34a、miR-181b、miR-195、miR-346、miR-432、miR-7、miR-132、and miR-212）的相对表达水平.结果 病例组血浆miRNA-132、miRNA-195、miRNA-30e、miRNA-7（5.06± 1.16、2.99± 1.27、1.88±1.62、9.59±1.85）较对照组（5.91±0.93、4.45±1.06、3.56± 1.30、11.8±2.53）显著上调（P＜0.05 ～ P＜0.001）.病例组外周血单核细胞miRNA-212、miRNA-34a、miRNA-30e（0.28±0.54、2.69± 1.10、-4.72± 1.01）较对照组（0.84±0.64、3.97±1.01、-3.55±1.26）同样显著上调（P＜ 0.05～ P＜0.001）.ROC曲线分析显示,miR-30e在血浆和单核外周血中均具有较高诊断价值.从血浆提取的microRNA-30e的AUC是0.767 （95％CI：0.608～0.926）,敏感度90.90％,特异度60.00％;从外周血单核细胞提取的miRNA-30e的AUC是0.756（95％ CI：0.584～0.929）,敏感度81.80％,特异度68.00％.结论 精神分裂症患者外周血血浆及单核细胞中microRNA的表达水平不同,无明显的相关性.miR-30在血浆和单核外周血中具有较高的诊断价值.

Partial microRNA profiling differences between peripheral plasma and mononuclear leucocytes in schizophrenia patients

Objective To explore the differences of microRNA expression as specific blood biomarker between peripheral plasma and mononuclear leucocytes in schizophrenia patients.Methods A total of 60 schizophrenia patients and 72 healthy controls were enrolled into our study,and nine most reported schizophrenia-associated microRNAs （miR-30e,miR-34a,miR-181 b,miR-195,miR-346,miR-432,miR-7,miR-132 and miR-212） expression levels were tested in peripheral plasma and mononuclear leucocytes using real-time PCR.Results MiRNA-132,miRNA-195,miRNA-30e and miRNA-7 （5.06± 1.16,2.99± 1.27,1.88± 1.62,9.59± 1.85） showed significantly higher expression in plasma samples（5.91±0.93,4.45±1.06,3.56± 1.30,11.8±2.53） （P＜0.05,P＜0.001） ; miRNA-212,miRNA-34a and miRNA-30e（0.28±0.54,2.69± 1.10,-4.72± 1.01） also showed significantly higher expression in mononuclear leucocytes samples （0.84±0.64,3.97± 1.01,-3.55 ± 1.26） （P＜0.05,P＜0.001）.ROC curve analysis suggested that the AUC of microRNA-30e in plasma was 0.767 （95％ CI：0.608～0.926） with sensitivity of 90.90％ and specificity of 60.00％,and the AUC of microRNA-30e in mononuclear leucocytes was 0.756 （95％ CI：0.584-0.929） with sensitivity of 81.80％ and specificity of 68.00％.Conclusion The microRNA expression in plasma varies greatly with that in mononuclear leucocytes.MicroRNA-30e can serve as specific bloodbased biomarker for schizophrenia diagnosis.