肺鳞癌患者和健康人血清的差异蛋白质组学研究

目的：比较肺鳞癌患者和健康人血清蛋白质组的差异，为筛选肺鳞癌血清分子标志物提供依据。方法：以5例健康人和5例I期肺鳞癌患者的血清为样本，采用15%的聚丙稀酰胺凝胶电泳(SDS-PAGE)分离血清蛋白质，Bandleader凝胶图像分析软件识别两种血清样本差异的蛋白质条带，电喷雾-四极杆-串联质谱（ESI-Q-TOF MS/MS）鉴定差异蛋白质条带中的蛋白质。Western印迹检测差异蛋白质结合珠蛋白-2（haptoglobin-2，HP-2)在肺鳞癌患者和健康人血清中的表达。结果：建立了健康人和肺鳞癌患者血清样品的一维凝胶电泳图谱，图像分析识别了4条差异蛋白质条带，质谱鉴定出29种非冗余的血清蛋白质。Western印迹证实了HP-2在肺鳞癌患者和健康人血清中的差异表达。结论：29种血清差异蛋白质为筛选肺鳞癌的血清分子标志物提供了实验依据。

Differential proteomic analysis of sera from lung squamouscarcinoma patients and healthy subjects

Objective To compare the serum proteome difference of lung squamous carcinoma(LSC) patients and healthy subjects,and to provide experimental evidence for finding serum biomarkers of LSC.Methods 15% SDS-PAGE were performed to separate serum proteins from 5 healthy subjects and 5 patients with stage 1 LSC,respectively.Bandleader software was applied to analyze 1-D gel images,and the differential serum protein bands between LSC patients and healthy subjects were identified by ESI-Q-TOF MS/MS.Western blot was used to detect the expression levels of differential protein Haptoglobin-2 in sera from LSC patients and healthy subjects.Resluts 1-D gel maps of serum proteins from LSC patients and healthy subjects were established.Four differential protein bands between the two types of sera were detected by image analysis,and a total of 29 non-redundant serum proteins in 4 differential protein bands were identified by MS.The differential expression levels of haptoglobin-2 in sera from LSC patients and healthy subjects were confirmed by Western blot.Conclusion Twenty-nine serum differential proteins will be useful to screening for serum biomarkers of lung squamous carcinoma.