p27kip1和cyclin D1在肾癌中的表达及临床意义

目的:研究肾癌组织中p27kip1和cyclinD1蛋白的表达,探讨其与肾癌生物学特征的内在联系。方法:应用免疫组化S-P法,对53例肾癌及6例正常肾组织中p27kip1和cyclinD1蛋白进行检测。结果:在正常肾组织中p27kip1均呈阳性表达,p27kip1阳性表达率在肾癌不同临床分期,不同病理分级中差异显著(G1～G2:70.00%,G3～G4:27.27%,P<0.05;T1～T2:70.83%,T3～T4:20.69%,P<0.05)。cyclin D1在正常肾组织呈阴性表达,cyclinD1阳性表达率在肾癌不同临床分期,不同病理分级中差异显著(G1～G2:51.35%,G3～G4:27.27%,P<0.05;T1～T2:58.33%,T3～T4:20.69%,P<0.05)。结论:p27kip1和cyclinD1蛋白在肾癌中的表达与肿瘤生物学行为密切相关,p27kip1和cyclinD1在肾癌的发生、发展中起重要作用。

Expression of p27kip1 and cyclin D1 in renal cell carcinoma and their clinical significance

Objective:To evaluate the expressions of p27kip1 and cyclin D1 in renal cell carcinoma(RCC),and to explore the association between the biological characteristics of RCC and the expressions of p27kip1 and cyclin D1.Methods:The expressions of p27kip1 and cyclin D1 were detected by immunohistochemical S-P method in 53 cases of RCC and 6 normal renal tissues.Results:In normal renal tissues,p27kip1 was positively expressed and cyclin D1 was negatively expressed.There were significant differences in the positive expression rates of both p27kip1 and cyclin D1 in renal cell carcinomas of different pathological grades and clinical stages(p27Kip1:G1 to G2 70.00%,G3 to G4 27.27%,T1 to T2 70.83%,T3 to T4 20.69%;cyclin D1:G1 to G2 51.35%,G3 to G4 27.27%,T1 to T2 58.33%,T3 to T4 20.69%;all P < 0.05).Conclusion:The expressions of p27kip1 and cyclin D1 are closely correlated with biological behavior of RCC.p27kip1 and cyclin D1 may play important roles in the occurrence and development of RCC.