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| 错配修复基因hMLH1在雌激素诱导结肠癌细胞凋亡中的作用 | |
| 引用本文： | 王亚婷,金鹏,卢俊宇,苏锡明,高巍,陆晓娟,盛剑秋.错配修复基因hMLH1在雌激素诱导结肠癌细胞凋亡中的作用[J].基础医学与临床,2012,32(4):342-345. |
| 作者姓名： | 王亚婷 金鹏 卢俊宇 苏锡明 高巍 陆晓娟 盛剑秋 |
| 作者单位： | 1. 北京军区总医院消化内科,北京100700;山西医科大学第二临床医学院,山西太原030001 2. 北京军区总医院消化内科,北京100700;北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京100005 3. 北京军区总医院消化内科,北京,100700 |
| 基金项目： | 国家自然科学基金，医学分子生物学国家重点实验室专项经费 |
| 摘 要： | 摘 要 目的：探讨错配修复基因hMLH1在雌激素诱导结肠癌细胞凋亡中的作用。方法：将含野生型hMLH1-cDNA的质粒转入hMLH1缺陷的人结肠癌细胞株HCT116，在不同浓度的雌激素干预下，分别以流式细胞仪和活细胞计数试剂盒（cell counting kit-8）法检测转染后细胞的凋亡率和活性。结果：与空载组相比，转染hMLH1后雌激素能明显诱导HCT116细胞活性降低，且凋亡率增加。结论:hMLH1参与雌激素诱导的结肠癌细胞株HCT116凋亡。 |
| 关 键 词： | 雌激素 错配修复基因 结肠癌 凋亡 |
The role of mismatch repair gene hMLH1 in estrogen-induced apoptosis of colon cancer cell HCT116 |
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| WANG Ya-ting , JIN Peng , LU Jun-yu , SU Xi-ming , GAO Wei , LU Xiao-juan , SHENG Jian-qiu.The role of mismatch repair gene hMLH1 in estrogen-induced apoptosis of colon cancer cell HCT116[J].Basic Medical Sciences and Clinics,2012,32(4):342-345. | |
| Authors: | WANG Ya-ting JIN Peng LU Jun-yu SU Xi-ming GAO Wei LU Xiao-juan SHENG Jian-qiu |
| Institution: | 1(1.Dept.of Gastroenterology,General Hospital of Beijing Military Region,Beijing 100700;2.Second Clinical Medical College, Shanxi Medical Universty,Taiyuan 030001;3.National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences, CAMS & PUMC,Beijing 100005,China) |
| Abstract: | Objective Exploring the role of mismatch repair gene hMLH1 played in the apoptosis of estrogen-induced colon cancer cell HCT116.Methods Transmit wild-type hMLH1-cDNA plasmid into hMLH1-deficient colon cancer cells(HCT116 cells) and then treat cells with different concentrations of estrogen.Detect the rate of apoptosis and the activity of cells with flow cytometry and cell counting kit-8 respectively.Results After transfection of hMLH1,estrogen induces the activity of HCT116 cells as 0.34 ± 0.06 that were significantly lower than those in no-load group 0.77 ± 0.17(P<0.001).However,the apoptosis rate increased from 9.75 ± 0.53 to 45.20 ± 2.63(P<0.001).Conclusions hMLH1 is involved in estrogen-induced apoptosis of colon cancer cell line HCT116. |
| Keywords: | estrogen mismatch repair colon cancer apoptosis |
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