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| 敲低错配修复基因hMLH1可导致人胚肾细胞株293t的微卫星不稳定 | |
| 引用本文： | 高巍,金鹏,陆晓娟,卢俊宇,王亚婷,盛剑秋.敲低错配修复基因hMLH1可导致人胚肾细胞株293t的微卫星不稳定[J].基础医学与临床,2012,32(4):346-349. |
| 作者姓名： | 高巍 金鹏 陆晓娟 卢俊宇 王亚婷 盛剑秋 |
| 作者单位： | 1. 北京军区总医院消化内科,北京100700;大连医科大学研究生院,辽宁大连116044; 2. 北京军区总医院消化内科,北京100700;北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京100005 3. 北京军区总医院消化内科,北京,100700 |
| 基金项目： | 国家自然科学基金，医学分子生物学国家重点实验室专项经费 |
| 摘 要： | 目的：探讨人胚胎肾细胞株293t中错配修复基因hMLH1的表达量与微卫星稳定性的关系。方法：构建针对hMLH1 基因的shRNA 表达载体，并干扰293t细胞hMLH1的表达，评价敲低hMLH1前后293t细胞微卫星状态的变化。结果：干扰组293t细胞相比对照组hMLH1表达明显敲低，微卫星状态由稳定变为不稳定。结论：293t细胞的hMLH1表达量与其微卫星状态密切相关，敲低hMLH1可导致293t细胞的微卫星不稳定。 |
| 关 键 词： | 错配修复基因 hMLH1 微卫星不稳定 RNA干扰 |
Knocking down mismatch repair gene hMLH1 may influence microsatillite stability in human embryonic kidney cell line 293t |
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| GAO Wei , JIN Peng , LU Xiao-juan , LU Jun-yu , WANG Ya-ting , SHENG Jian-qiu.Knocking down mismatch repair gene hMLH1 may influence microsatillite stability in human embryonic kidney cell line 293t[J].Basic Medical Sciences and Clinics,2012,32(4):346-349. | |
| Authors: | GAO Wei JIN Peng LU Xiao-juan LU Jun-yu WANG Ya-ting SHENG Jian-qiu |
| Institution: | 1(1.Dept.of Gastroenterology,General Hospital of Beijing Military Region,Beijing 100700;2.Dalian Medical University,Dalian 116044; 3.National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,CAMS & PUMC,Beijing 100005,China) |
| Abstract: | Objective To explore the ralations between expression of hMLH1 gene and microsatillites stability in human embryonic kidney cell line 293t.Methods A plasmid vector encoding shRNA targeting hMLH1(pGPU6/GFP/Neo-shMLH1) was constructed for knocking down hMLH1 gene in 293t cell line,and evaluated the microsatallite stability before and after knocking down.Results The expression of MLH1 in knocking down group was obviously knocked down in 293t cell,and the microsatallite states was changed from microsatallite stability into microsatallite instability.Conclusions Knocking down hMLH1 gene may lead to microsatallite instability in cell line 293t. |
| Keywords: | mismatch repair gene hMLH11 microsatallite instability RNA interference |
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