维甲酸诱导的小鼠神经管畸形模型中神经系统相关基因的表达情况分析

 摘要: 目的: 研究在全反式维甲酸诱导小鼠神经管畸形模型中神经系统相关基因（ASH2L,HDAC4,NSPC1与DOK5）的表达变化。方法: 取8只E8.5的C57/BL6孕鼠随机分为两组：对照组（4只）,模型组（4只）。对照组腹腔注射橄榄油,模型组腹腔注射全反式维甲酸;E13.5取胚胎。采用Real-time PCR检测两组小鼠脑和脊髓中ASH2L,HDAC4,NSPC1与DOK5的mRNA表达;采用Western Blotting技术检测两组小鼠脑和脊髓中ASH2L,HDAC4,NSPC1与DOK5的蛋白表达情况。结果：全反式维甲酸可诱导小鼠出现典型神经管畸形;与对照组相比,全反式维甲酸处理组的致畸胚胎脑和脊髓中,ASH2L HDAC4, NSPC1和DOK5的mRNA表达有所下降（P<0.05）;同时这些基因的蛋白水平也显著下降 (P<0.05)。结论：ASH2L,HDAC4,NSPC1和DOK5可能是抑制全反式维甲酸致神经管畸形的潜在基因。

Expression of nervous system related gene in retinoic acid induced neural tube defects of mice

Objective To study the expression of four nervous system related genes:ASH 2 L,HDAC4,NSPC1 and DOK5 in the all-trans retinoic acid induced mouse neural tube defect model.Methods Divide eight E8.5 C57/BL6 pregnant mice randomly into two groups:control group and experimental group.Control group was intraperitoneal injected by olive oil;the experimental group was intraperitoneal injected by all trans retinoic acid which was dissolved in olive oil;Embryos were taken at E13.5.The mRNA expression of ASH 2 L,HDAC4,NSPC1 and DOK5 in mouse brain and spinal cord was detected by real-time PCR;the protein expression was detected by Western blot.Results All trans retinoic acid can induce a typical neural tube defects in mice.Compared with the control group,the expression of mRNA and protein declined in fetal mice brain and spinal cord which is treated with all trans RA(P<0.05).Conclusions ASH 2 L,HDAC4,NSPC1 and DOK5 may be the underlying genes of inhibiting all trans RA induced neural tube defects.