肿瘤相关巨噬细胞microRNA表达谱及生物信息学分析

 目的 研究肿瘤相关巨噬细胞（TAM）microRNA的表达谱。 方法 建立小鼠乳腺癌细胞系4T1移植瘤模型，从移植瘤组织中分离TAM，用基因芯片检测microRNA表达谱，实时荧光定量PCR（Real-time PCR）验证芯片结果并进行生物信息学分析，以小鼠腹腔巨噬细胞（PEC）为阴性对照。 结果 与阴性对照细胞相比，TAM中有59个microRNAs表达量出现显著变化，其中23个microRNAs表达上调，有36个microRNAs表达下调；实时荧光定量PCR对miR-146a、miR-222、miR-31和miR-877的表达进行了验证，其结果与基因芯片检测结果一致；这些microRNAs参与了多个信号通路的调控。 结论 microRNA表达谱及生物信息学分析表明microRNA在TAM分化过程的调控中有重要作用。

Profile of microRNA expression in tumor associated macrophage and bioinformatics analysis

Objective To investigate the profile of microRNA expression in tumor associated macrophage (TAM). Methods An xenograft mouse model was established by using mouse breast cancer cell line 4T1. TAM were isolated from the tumor tissue. The microRNA expression profile was detected by using a microRNA chip assay. The result of chip assay was validated by real-time PCR and analyzed by bioinformatics. The peritoneal macrophage was used as control. Results There were significant changes in 59 microRNAs’ expression in TAM compared with the negative control. Among these microRNAs, 23 microRNAs’ expression was up regulated and 36 microRNAs down regulated. These microRNAs participate in the regulation of various signaling pathways. Conclusion Profile of microRNA expression and bioinformatics analysis suggested microRNA plays an important role in regulation of TAM differentiation.