白介素-17对急性肺损伤小鼠中性粒细胞凋亡的影响

目的白介素-17（IL-17）作为前炎症因子可以导致趋化性细胞因子的增高,从而促进中性粒细胞和单核细胞的募集。本实验通过建立急性肺损伤（ALI）小鼠模型观察IL-17在体内环境下对中性粒细胞凋亡的影响,探讨体内环境下IL-17的变化与血液中性粒细胞的凋亡的关系。方法雄性C57小鼠30只,体质量22～27g,随机分为正常对照组（对照组）,15只;ALI模型组（模型组）,15只,脂多糖（LPS）腹腔注射6mg/kg、10ml/kg致急性肺损伤。分别观察小鼠呼吸频率、活动情况及直接光镜观察肺组织血管及肺泡变化情况,中性粒细胞凋亡,淋巴细胞CD3＋/4＋/8＋分类检测,血清IL-17含量的检测。结果急性肺损伤时小鼠的IL-17的含量（0.10±0.002）pg/L,正常对照组（0.08±0.003）pg/L（P〈0.05）。CD4＋%模型组（21.99±1.83）,对照组（14.97±0.99）（P〈0.01）。中性粒细胞凋亡百分比（%）模型组（1.15±0.17）%,对照组（0.32±0.05）%（P〈0.01）。结论在ALI发病24h时,由于各种炎性细胞因子及其他炎性介质的作用,中性粒细胞表达了较高的凋亡率,而IL-17在其中起到了一定作用,中性粒细胞的凋亡与IL-17有一定的相关性。

Effect of Interleukin-17 on PMN apoptosis of acute lung injury mice

Objective As pro-inflammatory cytokine（PIC）,Interleukin（IL-17） can lead the increase of chemoattractant cytokine,which induce the recruitment of PMN and monocytes. Through the setting of ALI mice model in the study,to observe the effect of Interleukin（IL-17） on PMN apoptosis in vivo,and the relation between in vivio change of IL-17 and PMN apoptosis. Methods 30 male C57 mices,with weight of 22 ～ 27 g were divided randomly to normal control group（ control group）,15 mices; ALI model group（ Model group）,10 mices,LPS intraperitoneal injection 6 mg/kg,10 ml/kg was made to induce acute lung injury. Respiratory rate and movement of mice was observered,light microscope observation was made for the change of lung vessels and alveolus,PMN apoptosis testing,Lymphocyte CD3 ＋ /4 ＋ /8 ＋ classified detection and detection of IL-17 concentration in serum was made respectively. Results IL-17concentration in mice with ALI was（0. 10 ± 0. 002） pg/L,normal control group（0. 08 ± 0. 003） pg/L （P〈0. 05）. CD4 ＋ % model group（21. 99 ± 1. 83） control group（14. 97 ± 0. 99）（P〈0. 01）. PMN apoptosis percentage（% ）model group（1. 15 ± 0. 17）% control group （0. 32 ±0. 05）%（P〈0. 01）. Conclusions At 24 hours after ALI occur,percentage of PMN apoptosis is relatively high,due to the effect of various inflammatory cytokines and other inflammatory medium,it is IL-17 that plays a certain role in the process,which indicates that PMN apoptosis is related to IL-17.