参芪扶正注射液对老龄大鼠脑缺血/再灌注损伤的保护作用

目的研究参芪扶正注射液对老龄大鼠脑缺血损伤后的保护作用。方法老年雄性SD大鼠,体重200～300g,随机分为模型组、假手术组、尼莫地平阳性对照组(简称尼莫地平组)和参芪扶正注射液组(简称参芪组)。用改良Longa法制作大鼠局灶性脑缺血/再灌注模型,参芪扶正注射液在缺血前1周静脉给药,观察缺血3h再灌注3h后对大鼠神经功能障碍、脑梗塞范围、血清乳酸脱氢酶(LDH)和肌酸激酶(CK)活性、脑组织钙、水、丙二醛(MDA)和超氧化物歧化酶(SOD)含量。结果参芪能明显改善大鼠神经功能缺陷,减少脑组织含水量,缩小脑梗塞范围,抑制Ca~(2+)聚集,血清中LDH、CK及脑组织中MDA含量明显低于模型组,脑组织中SOD活性明显高于模型组。结论参芪扶正注射液对老龄大鼠脑缺血损伤有保护作用,其机制可能与抑制脂质过氧化反应和抑制脑组织Ca~(2+)聚集有关。

Protective Effect of Shenqi Fuzheng Injection on Cerebral Ischemia/Reperfusion Injured Aged Rats

Objective To study the protective effects of Shenqi Fuzheng Injection (SFI) on cerebral ischemi- a/reperfusion injured aged rats.Methods Aged SD male rats,weighing 200-300 g,were randomly divided into 4 groups:the model group,the sham-operative group,the nimodipine positive control group (abbreviated as nimo- dipine group) and the SFI group.Focal cerebral ischemia/reperfusion injured rat model was established by modi- fied Longa method.SFI was administered by intravenous dripping 1 week before isehemia.Nervous function disor- der,brain infarction area,serum lactate dehydrogenase (LDH) and creatine kinase (CK) levels,brain contents of Ca~(2+),water,MDA and SOD levels were observed 3 hrs after ischemia and 3 hrs after reperfusion.Results Ex- perimental results showed that SFI could obviously improve the deficit of nerve function,decrease water content of brain,reduce the infarction area of brain,and inhibit Ca~(2+) aggregation.LDH and CK levels in serum and MDA in brain were obviously lower than those in the model group and SOD activity in cerebral tissue was obviously higher than that in the model group.Conclusion SFI had protective effect on cerebral ischemia/reperfusion injured aged rats,whose mechanism might be related to the inhibition of lipid peroxidation and Ca~(2+) aggregation.