Effects of the CDC2 gene on adaptive mutation in the yeast Saccharomyces cerevisiae |
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Authors: | Hanna Baranowska Zofia Policińska Witold J Jachymczyk |
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Institution: | (1) Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5a Pawi skiego Street, PL 02-106 Warsaw, Poland |
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Abstract: | We have studied the influence of a temperature-sensitive cdc2-1 mutation in DNA polymerase on the selection-induced mutation occurring at the LYS-2 locus in the yeast Saccharomyces cerevisiae. It was found that in cells plated on synthetic complete medium lacking only lysine, the numbers of Lys+ revertant colonies accumulated in a time-dependent manner in the absence of any detectable increase in cell number. When cdc2-1 mutant cells, after selective plating, were incubated at the restrictive temperature of 37°C for 5 h daily for 7 days, the frequency of an adaptive reversion of lys
- Lys+ was significantly higher than the frequency in cells incubated only at the permissive temperature, or in wild-type cells incubated either at 23°C or 37°C. Therefore, when the proof-reading activity of DNA polymerase is impaired under restrictive conditions, the frequency of adaptive mutations is markedly enhanced. |
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Keywords: | DNA polymerase " target="_blank">gif" alt="delta" align="BASELINE" BORDER="0"> CDC2 Adaptive mutation S cerevisiae |
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