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Rearrangements of t-cell receptor β-chain genes in human leukaemias |
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| Authors: | Sharad Kumar Martin F Lavin Peter J Smith Lucia Pemble Russell J Collins and Roger L Prentice |
| Institution: | * Department of Biochemistry, University of Queensland, Brisbane, Australia 4067 ? Queensland Institute of Medical Research, Brisbane, Australia 4029 § Division of Immunology, Royal Brisbane Hospital, Brisbane, Australia 4029 |
| Abstract: | Rearrangements of the T-cell receptor (TCR), β-chain genes and immunoglobulin (Ig) heavy chain genes in several T-cell leukaemias (T-ALL and ATL), and some B-cell and myelogenous leukaemias were investigated. Two out of 15 cases of T-cell leukaemia tested failed to show a rearrangement pattern of TCR β genes although both expressed mRNA for this gene. The remaining 13 cases showed diverse patterns of rearrangements involving either Cβ1, Cβ2 or both. Cβ1, but not Cβ2 was deleted in s the T-cell leukaemias. Polyclonal T cells from four normal individuals showed the germ line pattern and an additional two bands in Hind III digested DNA. Except for one, all cases of C-ALL (B-cell leukaemia) showed a rearranged JH locus which was not evident in any of T-cell leukaemias studied. One case of B-cell leukaemia showed a rearrangement of both TCR β genes and JH genes. The results of these studies suggest that rearrangement of TCR and Ig genes occurs at a very early stage of differentiation of stem cells and does not appear to play a direct role in leukaemogenesis per se. |
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