软肝宁煎剂对肝纤维化大鼠防治作用的实验研究

目的观察软肝宁煎剂防治肝纤维化的作用并探讨其作用机制。方法采用CCl4复合因素诱导Wistar大鼠建立肝纤维化模型，设正常对照组、肝纤维化模型组、软肝宁煎剂预防组和软肝宁煎剂治疗组。实验结束后分别测定各组大鼠的肝脏指数和肝功能：ALT、AST，肝纤维化指标，透明质酸（HA），并作肝组织病理学检测及肝组织中转化生长因子（TGF—β1）免疫组化染色。结果1．软肝宁煎剂预防组、治疗组与模型组肝纤维化大鼠组织学比较有显著性差异（P〈0．05，P〈0．01）；2．在肝脏指数方面：预防组与模型组比较有显著性差异（P〈0．05），而治疗组无明显差异；3．软肝宁煎剂能明显降低大鼠血清中ALT、AST和大鼠肝脏组织中HA的含量，与模型组比较有显著性差异（P〈0．01）；4．软肝宁煎剂预防组和治疗组与模型组比较，明显抑制了TGF—β1的表达。结论软肝宁煎剂可通过保护肝细胞，减轻肝细胞损伤，促进ECM的降解，减少细胞因子TGF—β1的产生及释放来防治大鼠肝纤维化的发生和发展。

Experimental Research of Ruanganning Decoction on Prevention and Treatment of Hepatic Fibrosis in Rats

Objective To observe the function of Ruanganning Decoction and explore its mechanism on prevention and treatment of hepatic fibrosis.Methods CCl4 compound factor was used to induce and set up hepatic fibrosis model in Wistar rats.Normal control group,hepatic fibrosis model group,Ruanganning Decoction prevention group and Ruanganning treatment group were designed.After the end of experiment,liver indexes and liver functions of rats were determined in each group:ALT,AST,index of hepatic fibrosis and hyaluroni...