BACTEC MGIT960在二线抗结核药物药敏试验应用中的效果评价

目的 评价BACTEC MGIT 960进行4种二线抗结核药物药敏试验的效果.方法 用MGIT 960对结核分枝杆菌临床分离菌株进行二线抗结核药物卷曲霉素、卡那霉素、氧氟沙星和乙硫异烟胺的药敏检测,并将结果与L-J比例法结果进行比较分析.结果 111 株结核分枝杆菌临床分离株用MGIT960法与L-J比例法卷曲霉索、卡...

Evaluation of applying BACTEC MGIT 960 in drug susceptibility test to second-line anti-tuberculosis drugs

Objective To evaluate the effects of the BACTEC MGIT 960 in detecting the resistance of clinical Mycobacterium tuberculosis isolates to the second-line anti-tuberculosis drugs.Methods The drug susceptibility test of the clinical isolates to four anti-tuberculosis drugs,capreomycin（CPM）,kanamycin（KAN）,ofloxacin（OFLX） and Ethionamide（ETH） was conducted by MGIT 960,and the results were compared with those obtained by L-J proportion method.Results A total of 111 clinical Mycobacterium tuberculosis isolates were detected.The consistency of the drug susceptibility tests by MGIT 960 and L-J proportion method was 95.5% for CPM（106/111）,93.7% for KAN（104/111）,97.3% for OFLX（108/111） and 85.6% for ETH（95/111）.Compared with L-J proportion method,the sensitivity,specificity and accuracy of MGIT 960 were 80.0%,97.0%,95.5% for CPM,92.3%,93.9%,93.7% for KAN,95.3%,98.5%,97.3% for OFLX and 100%,81.2%,85.6% for ETH.The completion of the drug susceptibility test by MGIT 960 needed 8.3 days averagely,and that by L-J proportion method needed 26.2 days,the difference was statistical significant（P〈0.01）.Conclusion The result of drug susceptibility test by MGIT 960 was highly consistent with that by L-J proportion method,but the former needs much shorter time than the latter.The application of MGIT 960 could facilitate the early diagnosis and treatment of drug-resistant tuberculosis.