| Pax-8基因敲除小鼠心脏中NR4A1基因表达上调 |
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| 引用本文： | 黄晓燕,高瞻,周希,梁万前,陈锡文,陈通克,杨德业. Pax-8基因敲除小鼠心脏中NR4A1基因表达上调[J]. 温州医学院学报, 2011, 41(3): 205-208 |
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| 作者姓名： | 黄晓燕 高瞻 周希 梁万前 陈锡文 陈通克 杨德业 |
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| 作者单位： | 1. 温州医学院附属第一医院,心内科,温州医学院,心血管生物和基因研究所,浙江温州,325000
2. 温州医学院实验动物中心,浙江温州,325035 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的：寻找先天性心脏病室间隔缺损相关基因——转录因子Pax-8保护细胞凋亡的途径。方法：提取Pax-8基因敲除小鼠纯合子（Pax-8 KO-/-）和杂合子（Pax-8 KO＋/-）的心脏总RNA,利用小鼠基因的基因芯片检测两组小鼠基因表达水平,找出差异表达的基因,并经半定量RT-PCR和荧光实时定量PCR技术初步筛选出转录因子Pax-8的下游基因。结果：基因芯片检测发现,与Pax-8 KO＋/-组相比,在Pax-8 KO-/-小鼠中有25个基因表达下调,17个基因表达上调。用半定量RT-PCR验证发现,nuclear receptor sub-family4,group A,member 1（NR4A1）基因在Pax-8 KO-/-组上调。定量RT-PCR亦证实在Pax-8 KO-/-组NR4A1基因的表达水平较Pax-8 KO＋/-组及Pax-8＋/＋（野生型）组分别上调1.53倍和4.79倍（P〈0.01）。结论：NR4A1基因受Pax-8基因调控,在Pax-8保护细胞凋亡途径中发挥作用。
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| 关 键 词： | NR4A1 室间隔缺损 细胞凋亡 pairedboxgene8 小鼠 |
Up-regulation of NR4A1 gene expression in Pax-8 gene knockout mice myocardium |
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| HUANG Xiaoyan,GAO Zhan,ZHOU Xi,LIANG Wanqian,CHEN Xiwen,CHEN Tongke,YANG Deye. Up-regulation of NR4A1 gene expression in Pax-8 gene knockout mice myocardium[J]. Journal of Wenzhou Medical College, 2011, 41(3): 205-208 |
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| Authors: | HUANG Xiaoyan GAO Zhan ZHOU Xi LIANG Wanqian CHEN Xiwen CHEN Tongke YANG Deye |
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| Affiliation: | HUANG Xiaoyan*,GAO Zhan,ZHOU Xi,LIANG Wanqian,CHEN Xiwen,CHEN Tongke,YANG Deye.*Department of Cardiology of the First Affiliated Hospital,Institute of Cardiovascular Biology & Gen,Wenzhou Medical College,Wenzhou,325000 |
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| Abstract: | Objective: To find the possible genic pathway for the anti-apoptosis function of the ventricular septal defeet related to Pax-8.Method: The total RNA derived from the heart of Pax-8 KO-/-and Pax-8 KO+/-mice was extracted.Mouse genome DNA microarray was used to detect the differential expression level between the Pax-8 KO-/-and the Pax-8 KO+/-mice heart.The candidate genes were confirmed by RT-PCR and real time RT-PCR assay.Results: Microarray results showed that 25 genes were down-regulated and 17 were up-r... |
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| Keywords: | NR4A1 paired box gene 8 |
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